Regulatory Immunotherapy in Bone Marrow Transplantation

Figure 2

Phenotype of expanded iNKT cells. (a) PBMCs cultured for 7 days with α-GalCer in combination with rhIL-2 and rhIL-7 followed by FACS-based cell sorting for CD3⁺Vα24⁺ cells. Sorted iNKT cells were cultured with allo-PBMCs feeders, anti-CD3 mAb, and rhIL-2 and rhIL-7 for 21 days. (b) Cytokine profile of expanded (day 28) iNKT cells. Cellular cultures of FACS-sorted/expanded CD3⁺CD4^negVα24⁺ cells were stimulated for 5 hours with media alone or media + α-GalCer. Samples were analyzed using BD LSRII equipment and FlowJo 9.3.2 software. GrB: intracellular Granzyme B.

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