Regulatory Immunotherapy in Bone Marrow Transplantation

Figure 4

Ex vivo expansion of CD3⁺CD4⁺CD25⁺CD127^lo nTreg from adult peripheral blood (PB) and umbilical cord blood (UCB)-derived mononuclear cells. (a) Total cell numbers of cultured nTreg. FACS-sorted CD3⁺CD4⁺CD25⁺   T cells were cultured with Rapamycin and rhIL-2 for 21 days. (b) Fold expansion (from baseline absolute number at start of expansion) of ex vivo expanded nTreg from PB and UCB.

(a)

(b)