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The Scientific World Journal
Volume 2012, Article ID 105698, 8 pages
Research Article

Comparative Analysis of Apoptotic Resistance of Mesenchymal Stem Cells Isolated from Human Bone Marrow and Adipose Tissue

1Department of Cardiology, Faculty of Medicine, Bezmialem Vakif University, Adnan Menderes Bulvarı Vatan Caddesi, Fatih, 34093 Istanbul, Turkey
2Department of Cardiology, Faculty of Medicine, Kocaeli University, 41380 Kocaeli, Turkey
3Stem Cell and Gene Therapy Research and Applied Center, Faculty of Medicine, Kocaeli University, 41380 Kocaeli, Turkey

Received 13 October 2011; Accepted 5 January 2012

Academic Editors: F. A. Atik and G. Ristagno

Copyright © 2012 Gökhan Ertaş et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aim. Mesenchymal stem cells (MSCs) isolated from human bone marrow (hBM) and adipose tissue (hAT) are perceived as attractive sources of stem cells for cell therapy. The aim of this study was to compare MSCs from hBM and hAT for their immunocytochemistry staining and resistance to in vitro apoptosis. Methods. In our study, we investigated the antiapoptotic ability of these MSCs toward oxidative stress induced by hydrogen peroxide (H2O2) and serum deprivation. Results were assessed by MTT and flow cytometry. All experiments were repeated a minimum of three times. Results. Flow cytometry and MTT analysis revealed that hAT-MSCs exhibited a higher resistance toward H2O2-induced apoptosis (, hBM-hAT viability H2O2  , ) and to serum-deprivation-induced apoptosis at days 1 and 4 than the hBM-MSCs (, hAT-hBM absorbance, resp., day 1: , , day 4: , , and day 7: , ). hAT-MSCs showed superior tolerance to oxidative stress triggered by 2 mmol/L H2O2 and also have superior antiapoptosis capacity toward serum-free culture. Conclusion. In this study we found that hAT-MSCs are more resistant to in vitro apoptosis.