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The Scientific World Journal
Volume 2012, Article ID 128105, 9 pages
Research Article

Effect of Storage Conditions and Storage Periods on Seed Germination in Eleven Populations of Swertia chirayita: A Critically Endangered Medicinal Herb in Himalaya

Biodiversity Conservation and Management, G.B. Pant Institute of Himalayan Environment and Development, Sikkim Unit (Pangthang), P.O. Box 40, Gangtok, Sikkim 737 101, India

Received 30 October 2011; Accepted 14 December 2011

Academic Editors: M. Khan, S. Kikuchi, and W. Ramakrishna

Copyright © 2012 Bharat K. Pradhan and Hemant K. Badola. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Effect of different storage conditions (room temperature, 4°C, and −15°C) and different storage periods over 24 months on seed germination in Swertia chirayita collected from different altitudes in Sikkim Himalaya was determined. Multivariate ANOVA revealed significant ( 𝑃 < 0 . 0 0 0 1 ) effect of storage condition and storage period on seed germination and mean germination time. Seed germination percentage significantly ( 𝑃 < 0 . 0 1 ) varied between 87.78% (Sc5) and 100% (Sc2) during initial testing. Comparatively, high seed germination, low mean germination time, and low rate of fall in seed germination percentage in seeds stored at 4°C over different storage period were recorded. In addition, above 50% seed germination in majority of the populations even after 24 months of storage suggests 4°C as the most appropriate storage condition for long-term storage of seeds of S. chirayita.