Research Article

Effect of Storage Conditions and Storage Periods on Seed Germination in Eleven Populations of Swertia chirayita: A Critically Endangered Medicinal Herb in Himalaya

Table 1

General features and seed characteristics of different population of Swertia chirayita collected in Sikkim.

Name of populationsAltitude m aslNo. of seeds/fruit (10 fruits/population for seed counting)Seed weight (mg) 50 seeds × 3 replicates/populationSeed length (μm) 30 seeds ( 1 0 × 3 replicates)Seed width (μm) 30 seeds ( 1 0 × 3 replicates)Moisture content (%) 50 seeds × 3 replicates/populationHabitat

Sc1—Luing (ES)2126 2 0 9 ± 5 4 . 6 9 1 . 5 ± 0 . 0 6 4 8 6 ± 5 . 8 0 3 5 5 ± 1 2 . 2 9 2 5 . 8 ± 1 2 . 1 0 Ridge, moist grassy slope with Cryptomeria japonica and Castanopsis tribuloides.
Sc2—Railgaon (ES)1948 2 9 7 ± 7 6 . 9 7 1 . 6 ± 0 . 0 6 5 3 2 ± 1 3 . 9 4 3 8 3 ± 2 2 . 6 8 2 2 . 4 ± 3 . 2 7 Shrubberies with C. japonica and C. tribuloides.
Sc3—Upper Pangthang (ES)2176 3 0 9 ± 5 6 . 2 7 1 . 6 ± 0 . 1 0 5 0 2 ± 2 8 . 3 3 3 8 0 ± 1 1 . 5 5 2 9 . 1 ± 2 . 3 1 Shrubberies, moist grassy slope with Quercus lamellosa and C. tribuloides.
Sc4—Jaunbari (SS)1651 2 2 2 ± 4 6 . 3 4 1 . 2 ± 0 . 1 0 4 5 4 ± 1 4 . 2 2 3 7 4 ± 1 2 . 0 0 3 9 . 1 ± 6 . 0 8 Moist grassy slope with Alnus nepalensis and Michelia lanuginosa.
Sc5—Tiffin dara 2 (SS)1744 2 6 9 ± 2 5 . 2 5 1 . 4 ± 0 . 0 6 4 0 6 ± 8 . 0 5 3 6 2 ± 1 5 . 6 3 2 6 . 7 ± 7 . 8 2 Moist grassy slope with Cryptomeria japonica and Alnus nepalensis.
Sc6—Dhupi Dara (ES)2124 2 5 8 ± 2 9 . 0 1 1 . 8 ± 0 . 1 2 4 1 8 ± 1 3 . 1 1 3 7 8 ± 1 6 . 9 7 1 8 . 8 ± 1 . 9 7 Forest-Shrubberies with Cryptomeria japonica.
Sc7—Deewani Taar (WS)2055 2 9 4 ± 3 5 . 9 1 1 . 2 ± 0 . 1 0 3 5 8 ± 1 0 . 4 4 3 2 5 ± 1 0 . 6 9 3 6 . 5 ± 7 . 8 4 Forest-Shrubberies with the dominance of Alnus nepalensis.
Sc8—Gumpa Dara (WS)1987 2 6 0 ± 3 1 . 5 8 1 . 2 ± 0 . 0 6 3 7 2 ± 4 . 1 6 3 5 1 ± 4 . 5 8 2 9 . 7 ± 4 . 2 7 Forest-shrubberies with Cryptomeria japonica and Alnus nepalensis.
Sc9—Hilley (WS)2697 2 6 0 ± 3 3 . 5 5 1 . 4 ± 0 . 1 2 3 8 9 ± 9 . 5 4 3 6 9 ± 1 0 . 0 0 4 4 . 3 ± 3 . 7 1 Forest-Shrubberies with Lithocarpus pachyphylla and Arundinaria maling.
Sc10—Tendong (SS)2099 2 3 0 ± 3 3 . 0 7 1 . 6 ± 0 . 1 0 3 9 0 ± 1 0 . 9 7 3 7 2 ± 7 . 5 1 2 7 . 0 ± 2 . 2 3 Forest-Shrubberies with Castanopsis tribuloides and Quercus sp as dominant.
Sc11—Ravangla (SS)2160 2 5 6 ± 2 3 . 0 6 1 . 3 ± 0 . 0 0 3 2 0 ± 6 . 0 3 2 9 7 ± 3 . 2 1 2 5 . 6 ± 8 . 8 8 Mixed forest of Castanopsis tribuloides, Quercus lamellosa, Machilus edulis, and Cinnamomum tamala.

LSD ( 𝑃 < 0 . 0 5 )38.490.1421.5321.1410.54

𝐹 value5.2315.9576.9312.944.23

ES: East Sikkim; WS: West Sikkim; NS: North Sikkim; SS: South Sikkim; ± denotes the standard deviation.