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The Scientific World Journal
Volume 2012, Article ID 135921, 5 pages
Research Article

Effects of Exercise on Oxidative Stress in Rats Induced by Ozone

1Seción de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, IPN, Plan de San Luis y Salvador Diaz Mirón S/N, Colonia Casco de Santo Tomás, 11340 México, DF, Mexico
2Escuela Médico Militar, Department of Morphology, Boulevard Avila Camacho y Cerrada de Palomas S/N, Colonia Lomas de Sotelo, 11640 México, DF, Mexico
3Universidad Pablo de Olavide, Facultad del Deporte, Carretera de Utrera Km 1, Edificio 2, Planta Baja, 41013 Sevilla, Spain
4Kross-Link Laboratories, P.O. Box 374, Bellmore, NY 11710, USA

Received 30 October 2011; Accepted 22 December 2011

Academic Editor: Narisa Futrakul

Copyright © 2012 Catalina Martinez-Campos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Oxidative stress (OS) induced by acute exercise is reduced by chronic exercise. Ozone (O3) exposure produces OS. The aim of this study was to determine if aerobic exercise (AE) reduced OS produced by O3. A pilot experiment was performed with male Wistar rats submitted to AE (trained to swim 90 min/day). Adaptation to exercise was demonstrated three weeks after training by means of changes in reduced nitrates (NOx) in plasma. Therefore, two-week training was chosen for the following experiments. Six of twelve trained rats were exposed to O3 (0.5 ppm, 4 h/day, one hour before exercise). Two groups of sedentary animals ( 𝑛 = 6 each) were used as controls, one of which was exposed to O3. At the end of the experiments NOx, 8-isoprostane (8-IP), malondialdehyde (MDA), superoxide dismutase (SOD) activity, and carbonyls (CBs) were measured in plasma. CBs did not change in any group. O3-induced OS was manifested by reduced NOx and SOD activity, as well as increased 8-IP and MDA. Exercise significantly blocked O3 effects although SOD was also decreased by exercise (a greater drop occurring in the O3 group). It is concluded that AE protects against OS produced by O3 and the effect is independent of SOD.