A “Novel” Protocol for the Analysis of Hydroxycinnamic Acids in Leaf Tissue of Chicory (Cichorium intybus L., Asteraceae)

Table 1

Levels of caftaric, chlorogenic and chicoric acids in extracts obtained by the “novel” method and a “conventional” method. Similar amounts of tissue sampled from 2 comparable leaves from 3 different chicory plants were extracted by the two methods, and quantities of the hydroxycinnamate compounds in the extracts were determined by HPLC (see text for details). Content are expressed in μg/cm² of fresh leaf tissue area.

Sample	Compound	“Novel"		“Conventional"		Novel/conventional (%)
		𝜇g/cm^2	%	𝜇g/cm^2	%
1	Caf	7.1	26.2	7.6	33.4	92.6
	Chlo	5.8	21.7	5.8	25.2	101.4
	Chic	14.1	52.1	9.5	41.4	148.4
	Sum	27	100	22.9	100	117.9
2	Caf	6.8	25.1	7.6	33.2	89.9
	Chlo	6.2	22.9	5.7	24.8	109.7
	Chic	14.2	52.1	9.6	42	147.6
	Sum	27.2	100	22.8	100	119
3	Caf	13.8	19.1	16	28.7	86
	Chlo	15.8	21.9	16.6	29.9	94.6
	Chic	42.5	59	23	41.4	184.3
	Sum	72	100	55.7	100	129.2
4	Caf	13.9	18.9	16	28.6	87.2
	Chlo	15.7	21.2	16.7	29.9	93.8
	Chic	44.2	59.9	23.1	41.5	191.1
	Sum	73.8	100	55.8	100	132.2
5	Caf	11.3	17.9	7.8	18.7	145.5
	Chlo	16.8	26.5	13.9	33.2	121.2
	Chic	35.3	55.7	20.1	48.1	176.1
	Sum	63.5	100	41.7	100	152.1
6	Caf	11.4	17.9	8.8	20.3	128.8
	Chlo	16.8	26.4	14.2	32.5	118.8
	Chic	35.5	55.7	20.6	47.2	172.3
	Sum	63.7	100	43.6	100	146.1