Simple Reversed-Phase HPLC Method with Spectrophotometric Detection for Measuring Acetaminophen-Protein Adducts in Rat Liver Samples

Table 1

Results of a recovery study of APAP-Cys from spiked a rat liver matrix protein dispersion^a,b.

Amount added (μg)	Amount found (μg)	Mean recovery (%)
0.078	0.065 ± 0.003	83.3
0.156	0.136 ± 0.001	87.1
0.312	0.300 ± 0.002	88.5
0.625	0.547 ± 0.002	87.5
1.250	1.108 ± 0.002	88.6
2.500	2.262 ± 0.001	90.5
5.000	4.488 ± 0.002	89.8
10.000	8.906 ± 0.001	89.1
20.000	18.129 ± 0.001	90.6
40.000	36.380 ± 0.001	91.0

^aValues for amount found represent the mean ± SD for 𝑛=3 samples.
^bMean recoveries were calculated by comparing the peak area ratio responses of spiked rat liver samples with those of equipotent samples in distilled water and regarded as 100% recovery.