Simple Reversed-Phase HPLC Method with Spectrophotometric Detection for Measuring Acetaminophen-Protein Adducts in Rat Liver Samples

Table 2

Results of the intraday variability study on the proposed HPLC method based on three different concentrations of APAP-Cys added to distilled water and to blank rat liver matrix protein dispersion^a,b.

APAP-Cys injected (μg)	Peak area ratio; (mean ± SD, 𝑛=3)	RSD (%)
Samples in distilled water
0.156	0.005 ± 0.002	0.91
0.625	0.021 ± 0.001	0.72
1.250	0.042 ± 0.001	0.52
Samples in rat liver matrix protein dispersion
0.156	0.004 ± 0.001	1.02
0.625	0.018 ± 0.001	0.68
1.25	00.040 ± 0.003	0.63

^aEach sample was analyzed at 4 hr intervals three times on the same day.
^bRSD is the relative standard deviation.