Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep

Table 1

Antibodies used to analyze PBMC populations by flow cytometry.

Primary and secondary antibodies	Specificity	Ig isotype	Amount per tube (μg)	Source	Reference
Anti-sheep B lymphocytes	B lymphocytes	IgM	2	VMRD	BAQ44A
Anti-sheep CD4	T helper lymphocytes	IgG1	2	VMRD	17D1
Anti-sheep CD8	Cytotoxic T lymphocytes	IgG1	2	VMRD	CACT80C
Anti-sheep WC1	γδ subset of T lymphocytes	IgG1	2	VMRD	IL-A29
Anti-sheep CD25	IL-2 receptor α-chain	IgG1	2	VMRD	CACT116A
Anti-sheep CD14	Monocytes	IgG1	2	VMRD	CAM36A
FITC-conjugated anti-mouse IgG1 (γ1)	Mouse IgG1 (γ1)	—	0.4	Invitrogen	P-21129
PE-conjugated anti-mouse IgM	Mouse IgM	—	0.4	Sigma-Aldrich	F-9259