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The Scientific World Journal
Volume 2012 (2012), Article ID 149893, 5 pages
http://dx.doi.org/10.1100/2012/149893
Research Article

Defluorination of Sodium Fluoroacetate by Bacteria from Soil and Plants in Brazil

1Unidade Acadêmica de Medicina Veterinária, Universidade Federal de Campina Grande, Avenida Universitária, s/n, Bairro Sta. Cecília, Patos, PB, CEP: 58700-970, Brazil
2Laboratório de Fixação Biológica de Nitrogênio, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, CEP: 81531-980, Brazil
3Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, CEP: 50670-901, Brazil
4CSIRO Livestock Industries, Queensland Bioscience Precinct, Carmody Road, 306, St Lucia, 4067, QLD, Australia

Received 1 November 2011; Accepted 11 December 2011

Academic Editor: Fumihiko Takeuchi

Copyright © 2012 Expedito K. A. Camboim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this work was to isolate and identify bacteria able to degrade sodium fluoroacetate from soil and plant samples collected in areas where the fluoroacetate-containing plants Mascagnia rigida and Palicourea aenofusca are found. The samples were cultivated in mineral medium added with 20 mmol L−1 sodium fluoroacetate. Seven isolates were identified by 16S rRNA gene sequencing as Paenibacillus sp. (ECPB01), Burkholderia sp. (ECPB02), Cupriavidus sp. (ECPB03), Staphylococcus sp. (ECPB04), Ancylobacter sp. (ECPB05), Ralstonia sp. (ECPB06), and Stenotrophomonas sp. (ECPB07). All seven isolates degraded sodium-fluoroacetate-containing in the medium, reaching defluorination rate of fluoride ion of 20 mmol L−1. Six of them are reported for the first time as able to degrade sodium fluoroacetate (SF). In the future, some of these microorganisms can be used to establish in the rumen an engineered bacterial population able to degrade sodium fluoroacetate and protect ruminants from the poisoning by this compound.