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The Scientific World Journal
Volume 2012, Article ID 196347, 5 pages
Research Article

High Prevalence of Candida Yeast in Milk Samples from Cows Suffering from Mastitis in Poland

1Mycology Division, Preclinical Science Department, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-789 Warsaw, Poland
2Molecular Genetic Division, Mother and Child institute, Kasprzaka 17a, 01-211 Warsaw, Poland
3Division of Laboratory Diagnostics, Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland

Received 30 October 2011; Accepted 6 December 2011

Academic Editor: Lawrence W. Svenson

Copyright © 2012 Bozena Dworecka-Kaszak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Mastitis is an economically important disease in which fungi belonging to the genus Candida may participate as etiological agents. This study focused on determining the frequency of fungal isolation and differentiation of fungal species isolated from milk of mastitic cows. Sixty-six milk samples from mastitic cows were studied, and 55 strains of fungi were isolated. Seven different species classified as Candida were identified basing on phenotypic properties, and the dominating species was C. parapsilosis. Genomic DNA was isolated and amplified in PCR with ITS1 and NL2 primers. Amplification products were digested with restriction enzymes HpaII and EcoRI. Amplification of DNA with ITS1 and NL2 primers resulted in products of different sizes. Comparison of product sizes in restriction fragment PCR REA confirmed differences among species. Strains grouped together on the basis of phenotype characteristics differed in restriction fragment profiles. None of the investigated species showed similar genetic profiles.