Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples

Table 1

Determination of the robust incubation time range required to detect collection strains with the Milliflex Quantum method (𝑛=10).

Microorganism	Robust incubation time range	Fluorescence recovery ± SD; (%)	ANOVA 𝑃 value on fluorescence recoveries within robust incubation time range	Viability recovery ± SD (%)	ANOVA 𝑃 value on viability recoveries within robust incubation time range
Candida albicans ATCC 10231	22 h–26 h	102±38	0.66	101±39	0.75
Aspergillus brasiliensis ATCC 16404	28 h–32 h	99±18	0.71	97±19	0.66
Staphylococcus epidermidis ATCC 12228	14 h–18 h	98±18	0.20	101±15	0.28
Ralstonia pickettii ATCC 27511	22 h–26 h	91±26	0.33	92±26	0.34
Brevundimonas diminuta ATCC 19146	22 h–26 h	112±21	0.78	100±18	0.13
Staphylococcus aureus ATCC 6538	12 h–16 h	128±33	0.06	117±27	0.33
Pseudomonas aeruginosa ATCC 9027	16 h–20 h	111±28	0.91	103±24	0.16
Bacillus subtilis ATCC 6633	9 h–10 h	111±23	0.44	102±21	0.40
Escherichia coli ATCC 8739	8 h–10 h	122±29	0.06	111±24	0.44
Caulobacter sp. (environmental strain)	28 h–32 h	90±17	0.16	102±17	0.10

ANOVA, One-way analysis of variance; ATCC, American Type Culture Collection; SD, Standard deviation.