Biomedical Evaluation of Cortisol, Cortisone, and Corticosterone along with Testosterone and Epitestosterone Applying Micellar Electrokinetic Chromatography

Figure 1

Typical electropherograms obtained for (a) steroid-free urine sample; (b) male urine sample; (c) female urine sample; (d) urine sample spiked with 50 ng/mL of cortisol (1), 50 ng/mL of cortisone (2), 200 ng/mL of dexamethasone (3) (I.S.), 100 ng/mL of corticosterone (4), 100 ng/mL of testosterone (5), and 100 ng/mL of epitestosterone (6). Conditions of electrophoretic separation: 17 kV, injection 2 s, UV, λ = 254 nm, running electrolyte: 20 mM Na₂B₄O₇, 25 mM SDS.

(a)

(b)

(c)

(d)