Biomedical Evaluation of Cortisol, Cortisone, and Corticosterone along with Testosterone and Epitestosterone Applying Micellar Electrokinetic Chromatography

Table 2

Results of regression model for the analyzed steroids.

	Total cortisol	Total cortisone	Total corticosterone	Total testosterone	Total epitestosterone
Linearity range (ng/mL)	2–300
Slope ± SD	0.0062 ± 0.00013	0.0044 ± 0.00004	0.0077 ± 0.00008	0.0056 ± 0.00007	0.0067 ± 0.00005
Intercept ± SD	0.219 ± 0.0198	0.306 ± 0.0060	0.192 ± 0.0120	0.151 ± 0.0107	0.116 ± 0.0083
Correlation coefficient (𝑟)	0.9991	0.9998	0.9997	0.9997	0.9998
𝑛	6
LOD (ng/mL)	0.5
LOQ (ng/mL)	2.0
Total separation time (min)	8.0
	Migration time (min)
Cortisol	4.95
Cortisone	5.12
Corticosterone	5.97
Dexamethasone I.S.	5.55
Testosterone	6.48
Epitestosterone	6.66