Metabolic Syndrome, Hormone Levels, and Inflammation in Patients with Erectile Dysfunction

Arrabal-Polo, Miguel Ángel; Arias-Santiago, Salvador; López-Carmona Pintado, Fernando; Merino-Salas, Sergio; Lahoz-García, Clara; Zuluaga-Gómez, Armando; Arrabal-Martin, Miguel

doi:https://doi.org/10.1100/2012/272769

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Volume 2012 | Article ID 272769 | https://doi.org/10.1100/2012/272769

Metabolic Syndrome, Hormone Levels, and Inflammation in Patients with Erectile Dysfunction

Miguel Ángel Arrabal-Polo,¹Salvador Arias-Santiago,²Fernando López-Carmona Pintado,¹Sergio Merino-Salas,¹Clara Lahoz-García,¹Armando Zuluaga-Gómez,¹and Miguel Arrabal-Martin¹

Academic Editor: D. B. Vodusek, A. I. El Sakka, K. Akakura

Received26 Jun 2012

Accepted12 Aug 2012

Published02 Sept 2012

Abstract

Background. The end point of this study was to investigate the prevalence of MS in patients with ED in comparison with control subjects and to analyse the association with acute phase reactants (CRP, ESR) and hormone levels. Methods. This case-control study included 65 patients, 37 with erectile dysfunction, according to the International Index of Erectile Function (IIEF) from the Urology Department of San Cecilio University Hospital, Granada (Spain) and 28 healthy controls. The prevalence of metabolic syndrome was calculated according to ATP-III criteria. Hormone levels and acute phase parameters were studied in samples drawn. Results. The ATP-III criteria for MS were met by 64.9% of the patients with ED and only 9.5% of the controls (, OR = 17.53, 95% CI: 3.52–87.37). Binary logistic regression analysis showed a strong association between patients with ED and MS, even after additional adjustment for confounding factors (OR = 20.05, 95% CI: 1.24–32.82, ). Patients with hypogonadism presented a significantly higher prevalence of metabolic syndrome. Multiple linear regression analysis showed that systolic BP and CRP predicted 0.46 (model ) of IIEF changes. Conclusion. Chronic inflammation found in patients with ED might explain the association between ED and metabolic syndrome.

1. Introduction

Erectile dysfunction (ED) is defined as the inability to maintain an adequate erection for satisfactory sexual intercourse [1]. The degree of erectile dysfunction can be objectified by the international index of erectile function (IIEF), which allows us to classify ED as mild, moderate, or severe based on the score [2]. Related causes of erectile dysfunction are variable and can include vascular, endocrinological, neurological, and psychological causes [3]. The most common causes often involve alterations in the vascular endothelium due to atherosclerosis, which is a common physiopathological link between ED and cardiovascular disease (CD). Endothelial damage results in the reduced formation of nitric oxide, thereby decreasing blood flow, and negatively impacting erectile function [4]. Metabolic syndrome (MS) is associated with endothelial dysfunction and is defined by a number of components such as high blood pressure, elevated triglycerides, low HDL-cholesterol, increased abdominal circumference, or insulin resistance manifested as diabetes mellitus or glucose intolerance [5]. Metabolic syndrome is important because it may confer an overall cardiovascular risk that is higher than the individual components; subjects who met ATP-III MS criteria had a 2.59-fold greater likelihood (OR = 2.59) of experiencing a cardiovascular event in the next 10 years [6]. The severity of ED has been associated with a higher occurrence of major cardiovascular events and an increased risk of serious cardiovascular events [7]. Some inflammatory mediators, like C-reactive protein and fibrinogen, are elevated in patients with CD, especially in those with coronary heart disease. These mediators have clinical significance and could be useful in monitoring the treatment of these patients [8], but additional studies are necessary to confirm these results. Chronic inflammation plays an important role in the development of insulin resistance, endothelial dysfunction, and cardiovascular disease. Other studies report that plasma acute-phase protein levels are elevated in patients with ED (fibrinogen, von Willebrand factor, and interleukins) [9].

Recent studies have revealed that testosterone plays a protective role in the development of endothelial damage, and a negative linear relationship between testosterone levels and the severity of coronary disease has been found [10, 11]. Testosterone also plays an important role in erectile function and sexual desire, but it is not necessarily decreased in all patients with ED [10]. Different studies have shown that testosterone replacement therapy improves metabolic disease and central obesity [11].

The objectives of this study were to analyse the relationship between ED, MS, and systemic inflammation. The end point of this case-control study was to investigate the prevalence of metabolic syndrome in patients with ED in comparison with control subjects and to analyse the association with acute phase reactants (CPR, ESR, fibrinogen, D-dimer) and hormone levels.

2. Material and Methods

2.1. Patients and Controls

This case-control study included 65 outpatient males, 37 with erectile dysfunction consecutively selected, according to the International Index of Erectile Function (IIEF) from the Urology Department of San Cecilio University Hospital, Granada (Spain) and 28 healthy volunteers controls without erectile dysfunction from outside of the hospital. Participants from both groups answered the IIEF test to determine the presence (or not) of ED. The IIEF is a test with 15 questions about sexual activity, sexual desire, and sexual potency.

Inclusion criteria were male patients, age between 40 and 65 years with erectile dysfunction as defined by the IIEF and signing of the informed consent for study participation. Exclusion criteria were treatment based on an antiandrogen hormone therapy or drugs that cause iatrogenic erectile dysfunction (beta-blocking agents, antidepressants, antipsychotic drugs, etc.). Inclusion criteria for controls were age between 40 and 65 years and signing of the informed consent for study participation. Exclusion criteria for controls were the same as described above and the presence of ED.

2.2. Clinical and Laboratory Parameters

The severity of ED was determined by application of the IIEF test. The weight, height, and abdominal circumference of the subjects were measured, and their body mass index (BMI, kg/m²) was calculated. Systolic and diastolic blood pressure (BP) was measured after a 5 min rest and again 10 min later; the mean value was recorded.

Serum triglycerides (mg/dL), HDL-C (mg/dL), LDL-C (mg/dL), total cholesterol (mg/dL), glycaemia (mg/dL), insulin (uUI/mL), glycated haemoglobin (%) albumin (mg/dL), SHBG (nmol/L), total testosterone (ng/mL), free testosterone (ng/mL), bioavailable testosterone (ng/mL), FSH (mUI/mL), LH (mUI/mL), prolactin (ng/mL), progesterone (ng/mL), oestradiol (pg/mL), D-dimer (mg/L), fibrinogen (mg/dL), erythrocyte sedimentation rate (ESR, mm/h), and C-reactive protein (CRP, mg/dL) were studied in samples drawn between 8 and 9 a.m. after a rest period of ≥30 min. The homeostasis model assessment of insulin resistance (HOMA-IR index, μU/mg) was also calculated (fasting insulin × fasting glucose/22.5).

Data were also gathered on age, alcohol consumption (>40 g/day), smoking (>5 cigarettes/day), sedentarism (physical exercise <30 min/day), and drug intake (antihypertensives, diuretics, hypocholesterolemics or oral antidiabetics). The prevalence of metabolic syndrome was calculated according to ATP-III criteria. MS was defined by the presence of three of the following: abdominal circumference >102 cm; hypertriglyceridaemia >150 mg/dL, HDL-cholesterol <40 mg/dL, blood pressure >130/85 mmHg, or glycaemia >110 mg/dL.

Fasting blood samples were obtained between 8 and 9 a.m. after a rest period of ≥30 min by venipuncture of the patients’ large antecubital veins without stasis and after a 12-hour fasting period.

2.3. Statistical Analysis

Unpaired, two-sided Student’s t tests were applied to compare the mean values of quantitative variables, the Kolmogorov-Smirnov test to examine the normality of their distribution, and the Levene test to study the variance. The Mann-Whitney U test was used if the variables were not normally distributed. Qualitative variables were analysed with chi-square test or Fisher’s exact test if at least one cell had an expected count <5. Correlations among variables were studied by using the Pearson coefficient or Spearman’s methods if the variables were nonnormally distributed. Binary logistic regression models (the Wald method) were used to obtain estimated adjusted ORs and their 95% confidence intervals (CIs) to measure the association between ED and metabolic syndrome criteria. Multiple linear regression analysis was used to determine the independent predictors of IIEF, and the standardized coefficient of determination was calculated. Differences were considered significant at . The SPSS 17.0 programme was used for the data analyses (SPSS, Inc., Chicago, IL, USA).

2.4. Ethics

The study was approved by the Ethics Committee of San Cecilio University Hospital, and written informed consent was obtained from all patients and controls according to the Helsinki Declaration.

3. Results

The mean age of patients with ED was 55.8 ± 7.7 years versus 52.5 ± 4.8 years in the control group (). The mean BMI in the patient group was 29.8 ± 4.4 kg/m² versus 26.9 ± 4.6 kg/m² in the control group (). The IIEF score in patients was versus in the controls (). No significant differences were found between groups in terms of alcohol consumption (48.6% versus 64.3% for patients and controls, resp., ) or smoking (29.7% versus 42.8% for patients and controls, resp., ). However, 62.6% of patients admitted that they did not do any exercise versus 28.5% of controls (). A total of 29.7% of the patients with ED were previously diagnosed with arterial hypertension versus 25% in the control group (), and 32.4% of the patients presented with diabetes mellitus versus 10.7% of the controls ().

3.1. Metabolic Syndrome

ATP-III criteria for MS were met by 64.9% of the patients with ED versus 9.5% of the controls (, OR = 17.53, 95% CI: 3.52–87.37). Significant differences in MS parameters between ED patients and controls are listed in Table 1. In the group with ED, the abdominal circumference was cm versus cm in the control group (). Mean systolic and diastolic arterial blood pressure was mmHg and mmHg, respectively, in the patients and mmHg and mmHg in the controls (). Median glucose levels were 108 mg/dL in the group with ED versus 93 mg/dL in controls (), and median glycated haemoglobin was 5.8% versus 5.6% () for patients and controls, respectively. The MS criteria most frequently recorded in patients with ED were abdominal obesity and systolic and diastolic hypertension. A negative significant correlation between IIEF score and metabolic syndrome parameters was found: systolic BP (, ), diastolic BP (, ), abdominal perimeter (, ), and glucose levels (Spearman’s coefficient = −0.45, ). Multivariate studies with binary logistic regression showed a strong association between patients with ED and MS even after additional adjustment for age, BMI, IIEF, tobacco use, sedentarism, and alcohol consumption (OR = 20.05, 95% CI: 1.24–32.82, , Table 2).

3.2. Hormone Study

No significant differences in hormone levels were identified between patients and controls (Table 3). Patients were classified according to total testosterone levels to define the presence of hypogonadism (<3.5 ng/mL). Hypogonadism was found in 21.6% of patients with ED and in 8% of the control group. Patients with hypogonadism did not present with a significantly higher prevalence of ED (); however, patients with hypogonadism presented with a significantly higher prevalence of metabolic syndrome (77% of the patients with hypogonadism presented with metabolic syndrome versus 38.8% of the patients without hypogonadism, , OR = 2.75, 95% CI = 1.95–9.54). Negative significant correlations were observed between testosterone levels and weight (, ) and triglyceride levels (, ). A positive significant correlation was observed between testosterone levels and HDL-C (, ). Negative significant correlations between SHBG levels and weight (, ), abdominal perimeter (, ), and BMI (, ) were also found. In addition, there was a significant correlation between insulin levels and glycaemia (Spearman’s coefficient = 0.38, ) and between HOMA-IR and HDL-C (Spearman coefficient’s = −0.35, ).

3.3. Acute Phase Reactants

Table 4 shows the mean fibrinogen, D-dimer, ESR and CRP values observed in the study groups. Significant differences in C-reactive protein were found ( mg/dL versus mg/dL, resp., for patients and controls; ). No significant differences in median erythrocyte sedimentation rate were found (6 mm/h versus 5 mm/h for patients and controls, resp., ). Significant correlations between acute phase parameters and IIEF scores or metabolic syndrome criteria are shown in Table 5. Multiple linear regression analysis (Table 6) showed that systolic BP and CRP predicted 0.46 (model ) of IIEF changes (standardised for systolic BP: −0.58, and standardised for CRP: −0.22, ).

4. Discussion

The results of this study confirm the association between ED and higher cardiovascular risk. We found a higher prevalence of MS (ATP-III criteria) in patients with ED than in control subjects. Binary logistic regression showed a strong association between ED and metabolic syndrome after controlling for confounding variables. In addition, multiple linear regression analysis showed that systolic BP and CRP predicted IIEF changes in a high rate. No significant differences in hormone levels were identified between patients and controls, but patients with hypogonadism presented a higher prevalence of MS.

Diabetes mellitus, arterial hypertension, dyslipidaemia, obesity, and tobacco use are well known risk factors for cardiovascular disease because they are associated with endothelial dysfunction and the development of premature atherosclerosis. This endothelial dysfunction produces ED, due to reduced nitric oxide production and reduced vascular distensibility which inhibits an adequate erection during sexual intercourse [4, 12, 13]. ED has been linked in various studies with CD and MS [5, 6, 13].

In our study, patients with ED have a much higher prevalence of MS than the control group. These results are consistent with other results found in the literature which have shown that MS is strongly associated with ED [14–16]. We have observed that the MS criteria most frequently recorded in patients with ED were abdominal obesity and systolic and diastolic hypertension. These results are similar to those published by Bal et al., who reported that higher blood pressure, fasting glucose, and abdominal perimeter were the risk factors that best predicted the onset of ED [15]. Patients with ED and MS have a reduced elasticity in large arteries, resulting in a higher flow pressure and more arterial hypertension. Patients with ED and MS show a higher risk of cardiovascular events [17].

Some biochemical mediators have been measured in patients with suspected endothelial dysfunction [6, 9, 18]. In our study, we have observed that patients with ED had higher levels of C-reactive protein but not higher levels of fibrinogen and D-dimer or a higher erythrocyte sedimentation rate. A statistically significant negative linear correlation between IIEF score and C-reactive protein levels was found, which means that high levels of this mediator are related to the severity of ED. Elevated C-reactive protein levels in patients with ED have been found in patients with endothelial dysfunction [8].

In some patients with ED, the risk for CD and MS is associated with a deficit in testosterone levels [19, 20]. In our study, no significant differences were found in total, free, or bioavailable testosterone levels in patients with ED or the controls. Patients were divided into two groups according to their levels of total testosterone (lower than 3.5 ng/mL and higher to 3.5 ng/mL), and the prevalence of MS and ED in these two groups was analysed. Patients with hypogonadism did not present with higher rates of ED, but they did demonstrate a higher prevalence of MS. These results support the idea that MS may be associated with a deficiency of testosterone levels, and hormone replacement therapy may be useful in these patients [11, 21]. In addition, lower levels of testosterone are associated with higher weight and triglyceride levels and lower HDL-cholesterol, and SHBG correlated negatively with weight, body mass index and abdominal circumference [22–25]. We failed to demonstrate an association between testosterone levels and ED, thus we believe that testosterone does not play an important role in patients with ED when MS is present [10, 26–28]. We also believe that vascular endothelial changes are a common cause of ED [1].

In conclusion, the results obtained show that patients with ED have a higher prevalence of MS and higher mean values of acute phase parameters (CRP). Binary logistic regression analysis showed a strong association between ED and metabolic syndrome after controlling for multiple confounding variables. In addition, multiple linear regression analysis showed that systolic BP and CRP predicted a high percentage of IIEF changes. Cardiovascular screening by MS criteria assessment in patients with ED may be useful to detect individuals who are at risk and start preventive treatment against the development of cardiovascular disease.

Conflict of Interests

The authors declare that there is no conflict of interests.

Disclosure

The study was approved by the Ethics Committee of San Cecilio University Hospital.

Authors’ Contribution

All authors have participated and contributed in this paper.

Acknowledgment

The authors acknowledge Urology Department, San Cecilio University Hospital.

References

P. Cohan and S. G. Korenman, “Erectile dysfunction,” The Journal of Clinical Endocrinology & Metabolism, vol. 86, pp. 2192–2194, 2001.
View at: Google Scholar
R. C. Rosen, A. Riley, G. Wagner, I. H. Osterloh, J. Kirkpatrick, and A. Mishra, “The international index of erectile function (IIEF): a multidimensional scale for assessment of erectile dysfunction,” Urology, vol. 49, no. 6, pp. 822–830, 1997.
View at: Publisher Site | Google Scholar
R. V. Navarrete, J. G. Muñoz, L. L. Martín, J. V. G. Cardoso, and C. G. Enguita, “Metabolic sindrome and erectile dysfunction,” Archivos Españoles de Urologia, vol. 63, no. 8, pp. 673–678, 2010.
View at: Google Scholar
A. T. Guay, “ED: Erectile Dysfunction = Endothelial Dysfunction,” Endocrinology and Metabolism Clinics of North America, vol. 36, no. 2, pp. 453–463, 2007.
View at: Publisher Site | Google Scholar
G. Corona, E. Mannucci, G. Forti, and M. Maggi, “Hypogonadism, ED, metabolic syndrome and obesity: a pathological link supporting cardiovascular diseases,” International Journal of Andrology, vol. 32, no. 6, pp. 587–598, 2009.
View at: Publisher Site | Google Scholar
G. Assmann, H. Schulte, and U. Seedorf, “Cardiovascular risk assessment in the metabolic syndrome: results from the Prospective Cardiovascular Munster (PROCAM) study,” International Journal of Obesity, vol. 32, supplement 2, pp. S11–S16, 2008.
View at: Publisher Site | Google Scholar
G. Corona, M. Monami, V. Boddi et al., “Male sexuality and cardiovascular risk. a cohort study in patients with erectile dysfunction,” Journal of Sexual Medicine, vol. 7, no. 5, pp. 1918–1927, 2010.
View at: Publisher Site | Google Scholar
G. J. Blake and P. M. Ridker, “C-reactive protein and other inflammatory risk markers in acute coronary syndromes,” Journal of the American College of Cardiology, vol. 41, no. 4, supplement, pp. S37–S42, 2003.
View at: Publisher Site | Google Scholar
C. Vlachopoulos, K. Aznaouridis, N. Ioakeimidis et al., “Unfavourable endothelial and inflammatory state in erectile dysfunction patients with or without coronary artery disease,” European Heart Journal, vol. 27, no. 22, pp. 2640–2648, 2006.
View at: Publisher Site | Google Scholar
G. Corona and M. Maggi, “The role of testosterone in erectile dysfunction,” Nature Reviews Urology, vol. 7, no. 1, pp. 46–56, 2010.
View at: Publisher Site | Google Scholar
G. Corona, M. Monami, G. Rastrelli et al., “Testosterone and metabolic syndrome: a meta-analysis study,” Journal of Sexual Medicine, vol. 8, no. 1, pp. 272–283, 2011.
View at: Publisher Site | Google Scholar
R. Maas, E. Schwedhelm, J. Albsmeier, and R. H. Böger, “The pathophysiology of erectile dysfunction related to endothelial dysfunction and mediators of vascular function,” Vascular Medicine, vol. 7, no. 3, pp. 213–225, 2002.
View at: Publisher Site | Google Scholar
A. Müller and J. P. Mulhall, “Cardiovascular disease, metabolic syndrome and erectile dysfunction,” Current Opinion in Urology, vol. 16, no. 6, pp. 435–443, 2006.
View at: Publisher Site | Google Scholar
S. Heidler, C. Temml, C. Broessner et al., “Is the metabolic syndrome an independent risk factor for erectile dysfunction?” Journal of Urology, vol. 177, no. 2, pp. 651–654, 2007.
View at: Publisher Site | Google Scholar
K. Bal, M. Öder, A. S. Şahin et al., “Prevalence of metabolic syndrome and its association with erectile dysfunction among urologic patients: metabolic backgrounds of erectile dysfunction,” Urology, vol. 69, no. 2, pp. 356–360, 2007.
View at: Publisher Site | Google Scholar
R. Borges, P. Temido, L. Sousa et al., “Metabolic syndrome and sexual (Dys)function,” Journal of Sexual Medicine, vol. 6, no. 11, pp. 2958–2975, 2009.
View at: Publisher Site | Google Scholar
H. Pohjantähti-Maaroos and A. Palomäki, “Comparison of metabolic syndrome subjects with and without erectile dysfunction—levels of circulating oxidised LDL and arterial elasticity,” International Journal of Clinical Practice, vol. 65, no. 3, pp. 274–280, 2011.
View at: Publisher Site | Google Scholar
C. B. Eaton, Y. L. Liu, M. A. Mittleman, M. Miner, D. B. Glasser, and E. B. Rimm, “A retrospective study of the relationship between biomarkers of atherosclerosis and erectile dysfunction in 988 men,” International Journal of Impotence Research, vol. 19, no. 2, pp. 218–225, 2007.
View at: Publisher Site | Google Scholar
G. Corona, E. Mannucci, A. D. Fisher et al., “Low levels of androgens in men with erectile dysfunction and obesity,” Journal of Sexual Medicine, vol. 5, no. 10, pp. 2454–2463, 2008.
View at: Publisher Site | Google Scholar
R. Shabsigh, S. Arver, K. S. Channer et al., “The triad of erectile dysfunction, hypogonadism and the metabolic syndrome,” International Journal of Clinical Practice, vol. 62, no. 5, pp. 791–798, 2008.
View at: Publisher Site | Google Scholar
G. Corona, G. Rastrelli, A. Morelli, L. Vignozzi, E. Mannucci, and M. Maggi, “Hypogonadism and metabolic syndrome,” Journal of Endocrinological Investigation, vol. 34, no. 7, pp. 557–567, 2011.
View at: Publisher Site | Google Scholar
S. Arias-Santiago, M. T. Gutiérrez-Salmerón, L. Castellote-Caballero, A. Buendía-Eisman, and R. Naranjo-Sintes, “Androgenetic alopecia and cardiovascular risk factors in men and women: a comparative study,” Journal of the American Academy of Dermatology, vol. 63, no. 3, pp. 420–429, 2010.
View at: Publisher Site | Google Scholar
S. Arias-Santiago, M. T. Gutiérrez-Salmerón, A. Buendía-Eisman, M. S. Girón-Prieto, and R. Naranjo-Sintes, “Hypertension and aldosterone levels in women with early-onset androgenetic alopecia,” British Journal of Dermatology, vol. 162, no. 4, pp. 786–789, 2010.
View at: Publisher Site | Google Scholar
S. Arias-Santiago, M. T. Gutiérrez-Salmerón, L. Castellote-Caballero, and R. Naranjo-Sintes, “Elevated aldosterone levels in patients with androgenetic alopecia,” British Journal of Dermatology, vol. 161, no. 5, pp. 1196–1198, 2009.
View at: Publisher Site | Google Scholar
S. Arias-Santiago, M. T. Gutiérrez-Salmerón, A. Buendía-Eisman, M. S. Girón-Prieto, and R. Naranjo-Sintes, “Sex hormone-binding globulin and risk of hyperglycemia in patients with androgenetic alopecia,” Journal of the American Academy of Dermatology, 2011.
View at: Publisher Site | Google Scholar
B. Somani, S. Khan, and R. Donat, “Screening for metabolic syndrome and testosterone deficiency in patients with erectile dysfunction: results from the first UK prospective study,” BJU International, vol. 106, no. 5, pp. 688–690, 2010.
View at: Publisher Site | Google Scholar
A. M. Traish, R. J. Feeley, and A. Guay, “Mechanisms of obesity and related pathologies: androgen deficiency and endothelial dysfunction may be the link between obesity and erectile dysfunction,” FEBS Journal, vol. 276, no. 20, pp. 5755–5767, 2009.
View at: Publisher Site | Google Scholar
O. Rajmil, M. Fernández, A. Blasco, J. A. Arrús, R. Montañes, and J. Rodríguez-Espinosa, “Association of nocturnal penile rigidity with testosterone, metabolic syndrome, and other variables: a prospective cross-sectional pilot study,” Actas Urologicas Españolas, vol. 35, pp. 459–467, 2011.
View at: Google Scholar

Copyright

Copyright © 2012 Miguel Ángel Arrabal-Polo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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