The Scientific World Journal / 2012 / Article / Tab 2

Research Article

Population Genetic Analysis of Lobelia rhynchopetalum Hemsl. (Campanulaceae) Using DNA Sequences from ITS and Eight Chloroplast DNA Regions

Table 2

(1) Name and sequence of primers used for the amplification and sequencing of target DNA regions; (2) Genbank accession numbers of representative sequences and some sequence characteristics of each DNA region.

Target regionPrimer namePrimer sequenceGBANNVSWLrTALeNVSe%VSeNPISe%PISe%PIVSe

ITS a ITS5F c
ITS4R d
5′-GGAAGGAGAAGTCGTAACAAGG-3′
5′-TCCTCCGCTTATTGATATGC-3′
FJ664108-9 11719 198 27.5517.125.8

trnTUGU-trn L b U A A 37258Fd
37820Rc
5′-TGCAATGCTCTAACCTCTGA-3′
5′-CGATTTTATCATTTATCTATCTCCAA-3′
FJ664110-12525479.0152.931.9

trnfMCAU-trn S b U G A 48992Fd
49584Rc
49595Fd
50079Rc
5′-GTAGCTCGCAAGGCTCATAAC-3′
5′-TTCTGGTGGGTATCCTTAATTCTC-3′
5′-ATACCCACCAGAAAGACTAATCCA-3′
5′-CCATCTCTCCGAAAGACAATTTTA-3′
FJ664119-201976 134 13.7313.223.1

petN-trn C b G C A 58099Fc
58955Rc
5′-CCCAAGCGAGACTTACTATATCCA-3′
5′-AAATCCTTTTTCCCCAGTTCAA-3′
FJ664115-63291206.9124.160.0

trnGUCC-trn R b U C U 10002Fc
10226Rd
5′-CTAGCCTTCCAAGCTAACGATG-3′
5′-GACCTCTGTCCTATCCATTAGACAAT-3′
FJ664121-2219542.1 42.1100.0

psbT-psbBb 26837Fd
27102Rc
5′-GAATGTATAAACCAATGCTTCC-3′
5′-GAATTTGGAGCATTCCAAAAACT-3′
FJ6641181205188.883.944.4

ClpP intron 2 b 111104Fd
111454Rc
5′-GCCTTCGCCATATGAAA-3′
5′-ATGATGGCTCCGTTGCT-3′
FJ664112131136 11.6 113.530.6

3trnKUUU-matKb1825Fc
2195Rd
5′-CGGAACTAGTCGGATGGAGT-3′
5′-GCTTCTTCTATTTCGCGTAGGT-3′
FJ6641132307268.5 165.261.5

psbD-trn T b G G U 53562Fd
54107Rc
5′-TGATCTGTAATCAAAGCAAGATAGTGA-3′
5′-CGGTAGAGTAAGCCCATGGTA-3′
FJ6641171468439.2 163.437.2

aPrimers’ original reference is White et al. [14]; bprimers were designed for this study; cprimers were used both for amplification and sequencing; dprimers were used for amplification only; ethe values were calculated based on aligned sequences of Lobelia rhynchopetalum and Lobelia erinus. GBAN: gene bank accession numbers of L. rhynchopetalum sequences. Note: in cases when there is more than one accession number for a given DNA region, the accession numbers are given in range. For example, FJ664108-9 represents two accession numbers (FJ664108 and FJ664109). NVSWLr: number of variable sites within L. rhynchopetalum; TAL: total aligned length; NVS: number of variable sites; %VS: percent variable sites; NPIS: number of parsimony informative sites; %PIS: percent parsimony informative sites; %PIVS: percent parsimony informative variable sites.

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