Population Genetic Analysis of Lobelia rhynchopetalum Hemsl. (Campanulaceae) Using DNA Sequences from ITS and Eight Chloroplast DNA Regions

Table 2

(1) Name and sequence of primers used for the amplification and sequencing of target DNA regions; (2) Genbank accession numbers of representative sequences and some sequence characteristics of each DNA region.

Target region	Primer name	Primer sequence	GBAN	NVSWLr	TAL^e	NVS^e	%VS^e	NPIS^e	%PIS^e	%PIVS^e
ITS ^a	ITS5F ^c; ITS4R ^d	5′-GGAAGGAGAAGTCGTAACAAGG-3′; 5′-TCCTCCGCTTATTGATATGC-3′	FJ664108-9	11	719	198	27.5	51	7.1	25.8
trnTUGU-trn LbUAA	37258F^d; 37820R^c	5′-TGCAATGCTCTAACCTCTGA-3′; 5′-CGATTTTATCATTTATCTATCTCCAA-3′	FJ664110-1	2	525	47	9.0	15	2.9	31.9
trnfMCAU-trn SbUGA	48992F^d; 49584R^c; 49595F^d; 50079R^c	5′-GTAGCTCGCAAGGCTCATAAC-3′; 5′-TTCTGGTGGGTATCCTTAATTCTC-3′; 5′-ATACCCACCAGAAAGACTAATCCA-3′; 5′-CCATCTCTCCGAAAGACAATTTTA-3′	FJ664119-20	1	976	134	13.7	31	3.2	23.1
petN-trn CbGCA	58099F^c; 58955R^c	5′-CCCAAGCGAGACTTACTATATCCA-3′; 5′-AAATCCTTTTTCCCCAGTTCAA-3′	FJ664115-6	3	291	20	6.9	12	4.1	60.0
trnGUCC-trn RbUCU	10002F^c; 10226R^d	5′-CTAGCCTTCCAAGCTAACGATG-3′; 5′-GACCTCTGTCCTATCCATTAGACAAT-3′	FJ664121-2	2	195	4	2.1	4	2.1	100.0
psbT-psbB^b	26837F^d; 27102R^c	5′-GAATGTATAAACCAATGCTTCC-3′; 5′-GAATTTGGAGCATTCCAAAAACT-3′	FJ664118	1	205	18	8.8	8	3.9	44.4
ClpP intron 2 ^b	111104F^d; 111454R^c	5′-GCCTTCGCCATATGAAA-3′; 5′-ATGATGGCTCCGTTGCT-3′	FJ664112	1	311	36	11.6	11	3.5	30.6
3′ trnKUUU-matK^b	1825F^c; 2195R^d	5′-CGGAACTAGTCGGATGGAGT-3′; 5′-GCTTCTTCTATTTCGCGTAGGT-3′	FJ664113	2	307	26	8.5	16	5.2	61.5
psbD-trn TbGGU	53562F^d; 54107R^c	5′-TGATCTGTAATCAAAGCAAGATAGTGA-3′; 5′-CGGTAGAGTAAGCCCATGGTA-3′	FJ664117	1	468	43	9.2	16	3.4	37.2

^aPrimers’ original reference is White et al. [14]; ^bprimers were designed for this study; ^cprimers were used both for amplification and sequencing; ^dprimers were used for amplification only; ^ethe values were calculated based on aligned sequences of Lobelia rhynchopetalum and Lobelia erinus. GBAN: gene bank accession numbers of L. rhynchopetalum sequences. Note: in cases when there is more than one accession number for a given DNA region, the accession numbers are given in range. For example, FJ664108-9 represents two accession numbers (FJ664108 and FJ664109). NVSWLr: number of variable sites within L. rhynchopetalum; TAL: total aligned length; NVS: number of variable sites; %VS: percent variable sites; NPIS: number of parsimony informative sites; %PIS: percent parsimony informative sites; %PIVS: percent parsimony informative variable sites.