Research Article

Population Genetic Analysis of Lobelia rhynchopetalum Hemsl. (Campanulaceae) Using DNA Sequences from ITS and Eight Chloroplast DNA Regions

Table 3

Analysis of molecular variance (AMOVA) at different levels based on the Kimura K2P distance method.

GroupSource of variationsdfSum of squaresVariance components% variationsFixation index

The Bale populationsAP2 4.380.34Va40.42 𝐹 S T : 0.40*
WP12 5.980.49Vb59.58
Total14 10.360.84

The Simien populationsAP4 6.160.24Va39.89 𝐹 S T : 0.40*
WP20 7.130.36Vb60.11
Total24 13.290.59

All populationsAP795.202.63Va85.39 𝐹 S T : 0.85*
WP32 14.400.45Vb14.61
Total39109.603.08

Two mountainsaAM184.34.46Va87.01 𝐹 S T : 0.87*
WM38 25.30.66Vb12.99
Total39109.65.12

Two geographic groupsAG184.294.39Va85.87FSC: 0.37*
APWG6 10.910.27Vb5.34FST: 0.91*
WP32 14.400.45Vc8.79FCT: 0.86*
Total39109.605.12

* 𝑃 -value < 0.001 (significance test at 10000 permutations). AP: among populations; WP: within populations; AM: among mountains; WM: within mountains; AG: among groups; APWG: among populations within groups. aAnalysis was based on sequences pooled from individuals within the same mountain system.