The Design of New Adjuvants for Mucosal Immunity to Neisseria meningitidis B in Nasally Primed Neonatal Mice for Adult Immune Response
Figure 6
Immunoblot reactivity showing IgG antibodies binding to OMPs of N. meningitidis. (a)(1)–(6) With OMPs of N. meningitidis (B: 4:,19,5.5,L379,1,8) 28 days after i.n. immunization in the presence of stx1 (a)(1) or stx2 (a)(2). Immunization-specific IgG antibody responses at 42 days, 7 days post the i.m prime-boost in the presence of stx1 (a)(5) and stx2 (a)(6) as adjuvants in serum. Immunoreactivity of normal sera used as a control in (1,4). In (b)(1,2), immunoblot with sera of BALB/c mice immunized with OMPs and toxoids. IgG reactivity with (1) OMPs and stx1 28 days after i.n. immunization (2) OMP and stx1 42 days, 7 days post the i.m prime-boost and (b)(3,4) immunoblot with sera of BALB/c mice immunized with OMPs and toxoids. IgG reactivity with (1) OMPs and stx2 28 days after i.n. immunization (2) OMPs and stx2 42 days, 7 days post the i.m prime-boost. Molecular weight marker is on the left of (a).
