Thyroid Hormone-Induced Cytosol-to-Nuclear Translocation of Rat Liver Nrf2 Is Dependent on Kupffer Cell Functioning

Figure 3

Gadolinium chloride (GdCl₃) administration is associated with enhancement of liver Keap1/Nrf2 ratios over values in T₃-treated rats. Determinations were performed at 2 h after T₃ administration in rats pretreated with GdCl₃ for 72 h. (a) Levels of cytosolic Keap1 protein (70 kDa) and β-actin (43 kDa); (b) levels of nuclear Keap1 protein and lamin A/C (65 kDa); (c) nuclear Keap1/Nrf2 content ratios and net effects of T₃ and GdCl₃ treatments (inset). Values shown represent means ± SEM for 4 to 6 rats per experimental group. Significance (𝑃<0.05; two-way ANOVA and the Newman-Keuls’ test) is indicated by the letters identifying each experimental group. Significance in the inset of (c) was calculated by Student’s t-test for unpaired data.

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