Thyroid Hormone-Induced Cytosol-to-Nuclear Translocation of Rat Liver Nrf2 Is Dependent on Kupffer Cell Functioning

Figure 5

Apocynin administration is associated with diminution of T₃-induced activation of liver Nrf2 signaling. Determinations were performed at 2 h after T₃ administration in rats pretreated with apocynin for 7 days. (a) Levels of cytosolic Nrf2 protein (68 kDa) and β-actin (43 kDa); (b) levels of nuclear Nrf2 protein and lamin A/C (65 kDa); (c) nuclear/cytosolic Nrf2 content ratios and net effects of T₃ and apocynin treatments (inset); (d) levels of heme oxygenase 1 (HO-1) protein (33 kDa). Values shown represent means ± SEM for 3 to 4 rats per experimental group. Significance (𝑃<0.05; two-way ANOVA and the Newman-Keuls’ test) is indicated by the letters identifying each experimental group. Significance in the inset of (c) was calculated by Student’s t-test for unpaired data.

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