Combined Effect of Subchondral Drilling and Hyaluronic Acid with/without Diacerein in Full-Thickness Articular Cartilage Lesion in Rabbits

Table 1

Histological criteria used to demonstrate tissue types (Dorotka’s criteria) [14].

Tissue type	Histologic criteria
Fibrous tissue	(i) Highly orientated type I collagen fibrous structure; (ii) Cells with elongated shape, not in lacunae
Hyaline cartilage	(i) Ground glass-like appearance of the matrix; (ii) Type II collagen staining (with variable intensity of the latter); (iii) Spherical chondrocytes in mature lacunae
Fibrocartilage	(i) Encompassed a wide range of tissues between fibrous tissue and hyaline cartilage, including fibrocartilage; (ii) Oval and spherical cell shapes with and without lacunae; (iii) Varying intensity of staining for proteoglycans and type I and II collagen; (iv) No hyaline appearance of the matrix
Articular cartilage	(i) Hyaline cartilage with columnar arrangement of the cells; (ii) Matrix staining typical of articular cartilage