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The Scientific World Journal
Volume 2012 (2012), Article ID 348246, 5 pages
http://dx.doi.org/10.1100/2012/348246
Research Article

Decreased Erythrocyte NA+,K+-ATPase Activity and Increased Plasma TBARS in Prehypertensive Patients

1Department of Physical Education, Midwest State University, Campus Irati, 84500-000 PR, Irati, Brazil
2Departamento de Educação Física e Saúde, Universidade de Santa Cruz do Sul, 96815-900 Santa Cruz do Sul, RS, Brazil
3Departamento de Biologia e Farmácia Laboratório de Biotecnologia e Genética, 164, 96815-900 Santa Cruz do Sul, RS, Brazil
4Pharmaceutical Science Master Degree Program, Midwest State University, Campus CEDETEG, 85040-080 Guarapuava, PR, Brazil
5Faculdade Campo Real, Biomedicina, 85015-240 Guarapuava, PR, Brazil
6Departamento de Educação Física, Universidade Federal do Paraná, 80215370 Curitiba, PR, Brazil

Received 19 March 2012; Accepted 17 May 2012

Academic Editors: M. Y. Arica, A. Capell, and P. Madsen

Copyright © 2012 Carlos Ricardo Maneck Malfatti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The essential hypertension has been associated with membrane cell damage. The aim of the present study is investigate the relationship between erythrocyte Na+,K+-ATPase and lipoperoxidation in prehypertensive patients compared to normotensive status. The present study involved the prehypertensive patients (systolic: 1 3 6 ± 7  mmHg; diastolic: 8 6 . 8 ± 6 . 3  mmHg; 𝑛 = 8 ) and healthy men with normal blood pressure (systolic: 1 1 0 ± 6 . 4  mmHg; diastolic: 7 6 . 1 ± 4 . 2  mmHg; 𝑛 = 8 ) who were matched for age ( 3 5 ± 4 years old). The venous blood samples of antecubital vein (5 mL) were collected into a tube containing sodium heparin as anticoagulant (1000 UI), and erythrocyte ghosts were prepared for quantifying Na+,K+-ATPase activity. The extent of the thiobarbituric acid reactive substances (TBARS) was determined in plasma. The statistical analysis was carried out by Student’s t-test and Pearson’s correlation coefficient. A 𝑃 < 0 . 0 5 was considered significant. The Na+,K+-ATPase activity was lower in prehypertensive patients compared with normotensive subjects (4.9 versus 8.0 nmol Pi/mg protein/min; 𝑃 < 0 . 0 5 ). The Na+,K+-ATPase activity correlated negatively with TBARS content ( 𝑟 = 0 . 6 ; 𝑃 < 0 . 0 5 ) and diastolic blood pressure ( 𝑟 = 0 . 8 4 ; 𝑃 < 0 . 0 5 ). The present study suggests that Na+,K+-ATPase activity reduction and elevation of the TBARS content may underlie the pathophysiological aspects linked to the prehypertensive status.