A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application
Table 1
Column temperatures study. Experimental results (peak area mean ± RSD) obtained from six separate runs of a standard sample (aqueous mixture of 8 water-soluble vitamins) at 4 different column temperatures. The last row of the table reports run time in minutes. HPLC conditions used were those reported in the main text.
Vitamin
Peak area [mAU] ± R.S.D. measured at 280 nm at column temperature of 20°C
Peak area [mAU] ± R.S.D. measured at 280 nm at column temperature of 25°C
Peak area [mAU] ± R.S.D. measured at 280 nm at column temperature of 30°C
Peak area [mAU] ± R.S.D. measured at 280 nm at column temperature of 40°C
