Table 1: Column temperatures study. Experimental results (peak area mean ± RSD) obtained from six separate runs of a standard sample (aqueous mixture of 8 water-soluble vitamins) at 4 different column temperatures. The last row of the table reports run time in minutes. HPLC conditions used were those reported in the main text.

VitaminPeak area [mAU] ± R.S.D. measured at 280 nm at column temperature of 20°CPeak area [mAU] ± R.S.D. measured at 280 nm at column temperature of 25°CPeak area [mAU] ± R.S.D. measured at 280 nm at column temperature of 30°CPeak area [mAU] ± R.S.D. measured at 280 nm at column temperature of 40°C

C 3 6 7 . 0 9 ± 0 . 0 3 3 3 3 . 9 4 ± 0 . 0 1 3 6 4 . 9 8 ± 0 . 0 4 1 4 3 . 6 8 ± 0 . 0 2
B1 1 6 8 . 7 8 ± 0 . 0 1 2 4 7 . 2 5 ± 0 . 0 3 1 6 9 . 6 6 ± 0 . 0 5 5 0 . 6 8 ± 0 . 0 6
B2 2 0 6 5 . 7 6 ± 0 . 0 7 1 6 8 1 . 8 3 ± 0 . 0 5 2 1 3 8 . 7 5 ± 0 . 0 2 3 7 5 . 4 7 ± 0 . 0 3
B3 8 8 . 1 5 ± 0 . 0 3 8 9 . 6 1 ± 0 . 0 5 9 2 . 6 0 ± 0 . 0 2 4 4 . 5 1 ± 0 . 0 8
B5 5 1 . 0 5 ± 0 . 0 3 5 0 . 7 3 ± 0 . 0 8 6 0 . 1 3 ± 0 . 0 3 2 2 . 1 9 ± 0 . 0 9
B6 8 8 9 . 0 6 ± 0 . 0 2 9 2 4 . 1 1 ± 0 . 0 6 8 7 8 . 7 2 ± 0 . 0 8 1 9 1 . 7 0 ± 0 . 0 7
B9 1 8 1 . 2 5 ± 0 . 0 6 1 5 6 . 7 1 ± 0 . 0 5 2 0 5 . 2 1 ± 0 . 0 2 7 3 . 2 0 ± 0 . 0 3
B12 3 1 0 . 1 0 ± 0 . 0 2 5 3 2 . 5 2 ± 0 . 0 3 7 3 8 . 6 6 ± 0 . 0 5 5 7 4 . 2 7 ± 0 . 0 7

Total analysis time [mins]25 min25 min35 min45 min