A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application

Table 6

Mean concentration of individual water-soluble vitamins (±SEM) in plasma samples obtained from 24 study participants. Elevate variability in individuals’ vitamin levels may explain high S.E.M.s in the case of B₁, B₅ and B₆.

	C (μmol L^−1)	B1 (nmol L^−1)	B2 (nmol L^−1)	B5 (nmol L^−1)	B6 (nmol L^−1)	B9 (nmol L^−1)	B12 (nmol L^−1)
Mean (𝑛=24)	44.7	51.3	15.6	32.4	73.3	8.5	5.7
S.E.M	16.3	27.6	5.5	32.7	37.9	4.3	1.7
Ref. value [1, 2]	24–84	9–44	6.2–39	N/A	7–52	3.1–18	1.9–3.5

Ref. values: reference intervals obtained from Malmauret et al. 2002 [1] and from Talwar et al. [2].