Mixed Sequence Reader: A Program for Analyzing DNA Sequences with Heterozygous Base Calling
Figure 2
Experimental confirmation of the Indel identified by Mixed Sequence Reader. A 9-bp insertion at chromosome 7:55249011 was detected by Mixed Sequence Reader from a directly sequenced PCR product. (a) PCR direct sequencing chromatography trace, (b) MSR results. The PCR products were cloned, and at least ten single colonies were analyzed by DNA sequencing. One plasmid contained the wild type sequence (plasmid 1); whereas the other plasmids contained an insertion of “GGGGGTTGT,” as shown in plasmid 2 (a). The insertion sequence is shown between two arrows.
