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The Scientific World Journal
Volume 2012 (2012), Article ID 369802, 10 pages
http://dx.doi.org/10.1100/2012/369802
Research Article

Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits

1Laboratorio de Genética, Universidad de Cádiz, Poligono Río San Pedro s/n, 11510 Puerto Real, Spain
2Department of Cell Biology, Faculty of Science, Charles University in Prague, Prague 2, Viničná 7, 12843 Prague, Czech Republic
3Instituto de Ciencias Marinas de Andalucía, Consejo Superior de Investigaciones Científicas, República Saharaui, no. 2, 11510 Puerto Real, Spain

Received 27 October 2011; Accepted 21 December 2011

Academic Editor: Sardana Fedorova

Copyright © 2012 Irma Sánchez-Ramos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Growth is a priority trait from the point of view of genetic improvement. Molecular markers linked to quantitative trait loci (QTL) have been regarded as useful for marker-assisted selection in complex traits as growth. Polymorphisms have been studied in five candidate genes influencing growth in gilthead seabream (Sparus aurata): the growth hormone (GH), insulin-like growth factor-1 (IGF-1), myostatin (MSTN-1), prolactin (PRL), and somatolactin (SL) genes. Specimens evaluated were from a commercial broodstock comprising 131 breeders (from which 36 males and 44 females contributed to the progeny). In all samples eleven gene fragments, covering more than 13,000 bp, generated by PCR-RFLP, were analyzed; tests were made for significant associations between these markers and growth traits. ANOVA results showed a significant association between MSTN-1 gene polymorphism and growth traits. Pairwise tests revealed several RFLPs in the MSTN-1 gene with significant heterogeneity of genotypes among size groups. PRL and MSTN-1 genes presented linkage disequilibrium. The MSTN-1 gene was mapped in the centromeric region of a medium-size acrocentric chromosome pair.