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The Scientific World Journal
Volume 2012, Article ID 373709, 4 pages
http://dx.doi.org/10.1100/2012/373709
Research Article

The Shaker Potassium Channel Is No Target for Xenon Anesthesia in Short-Sleeping Drosophila melanogaster Mutants

1Department of Anaesthesiology and Operrative Intensive Care Medicine, University Hospital Schleswig-Holstein, Campus Kiel, Schwanenweg 21, 24105 Kiel, Germany
2Institute of Clinical and Experimental Pharmacology, University Hospital Schleswig-Holstein, Campus Kiel, Arnold-Heller-Straße 3, Haus 30, 24105 Kiel, Germany
3Department of Zoophysiology, CAU Kiel, Olshausenstraße 40, 24098 Kiel, Germany

Received 13 March 2012; Accepted 27 April 2012

Academic Editors: C. Rivat and A. Zwerling

Copyright © 2012 C. Schaper et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Xenon seems to be an ideal anesthetic drug. To explore if next to the antagonism at the NMDA-receptor other molecular targets are involved, we tested the xenon requirement in short sleeping Drosophila shaker mutants and in 𝑛 𝑎 [ 𝑎 𝑟 3 8 ] . Methods. The Drosophila melanogaster strains wildtype Canton-S, 𝑛 𝑎 [ 𝑎 𝑟 3 8 ] , 𝑠 1 0 2 and 𝑠 𝑚 𝑛 𝑠 , were raised and sleep was measured. Based on the response of the flies at different xenon concentrations, logEC50 values were calculated. Results. The logEC50-values for WT Canton-S were 1.671 (1.601–1.742 95%-confidence intervall; 𝑛 = 2 3 8 ; P versus 𝑠 1 0 2 > 0,05), for 𝑠 𝑚 𝑛 𝑠 1.711 (1.650–1.773; 𝑛 = 2 4 2 ; P versus WT Canton-S > 0,05). The logEC50-value for 𝑠 1 0 2 was 1.594 (1.493–1.694; 𝑛 = 2 6 1 ; P versus 𝑠 𝑚 𝑛 𝑠 > 0.05). The logEC-value of 𝑛 𝑎 [ 𝑎 𝑟 3 8 ] was 2.076 (1.619–2.532; 𝑛 = 2 0 7 ; P versus 𝑠 𝑚 𝑛 𝑠 < 0.05, versus 𝑠 1 0 2 < 0.05, versus WT Canton-S < 0.05). P values for all shaker mutants were 𝑃 > 0 . 0 5 , while 𝑛 𝑎 [ 𝑎 𝑟 3 8 ] was found to be hyposensitive compared to wildtype (P < 0.05). Conclusions. The xenon requirement in Drosophila melanogaster is not influenced by a single gene mutation at the shaker locus, whereas a reduced expression of a nonselective cation channel leads to an increased xenon requirement. This supports the thesis that xenon mediates its effects not only via an antagonism at the NMDA-receptor.