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The Scientific World Journal
Volume 2012, Article ID 390672, 4 pages
Research Article

FAD2-DGAT2 Genes Coexpressed in Endophytic Aspergillus fumigatus Derived from Tung Oilseeds

1State key Laboratory of Forest Genetics and Tree Breeding, Chinese Academy of Forestry, Beijing 100091, China
2Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, China

Received 17 May 2012; Accepted 4 June 2012

Academic Editors: T. Hatano and C. Lu

Copyright © 2012 Yi-Cun Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Recent efforts to genetically engineer plants that contain fatty acid desaturases to produce valuable fatty acids have made only modest progress. Diacylglycerol acyltransferase 2 (DGAT2), which catalyzes the final step in triacylglycerol (TAG) assembly, might potentially regulate the biosynthesis of desired fatty acids in TAGs. To study the effects of tung tree (Vernicia fordii) vfDGAT2 in channeling the desired fatty acids into TAG, vfDGAT2 combined with the tung tree fatty acid desaturase-2 (vfFAD2) gene was co-introduced into Aspergillus fumigatus, an endophytic fungus isolated from healthy tung oilseed. Two transformants coexpressing vfFAD2 and vfDGAT2 showed a more than 6-fold increase in linoleic acid production compared to the original A. fumigatus strain, while a nearly 2-fold increase was found in the transformant expressing only vfFAD2. Our data suggest that vfDGAT2 plays a pivotal role in promoting linoleic acid accumulation in TAGs. This holds great promise for further genetic engineering aimed at producing valuable fatty acids.