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The Scientific World Journal
Volume 2012 (2012), Article ID 395741, 5 pages
http://dx.doi.org/10.1100/2012/395741
Research Article

Oxidative Stress Level in the Testes of Mice and Rats during Nickel Intoxication

1Physiology and Biochemistry Department, University of Physical Education, Avenue I.J. Paderewskiego 35, 51-612 Wroclaw, Poland
2Hygiene Department, Medical University, Ul. J. Mikulicza-Radeckiego 7, 50-368 Wroclaw, Poland
3Biochemistry and Biophysic Institute, Polish Academy of Sciences, Ul. Pawińskiego 5a, 02-106 Warsaw, Poland
4Forensic Medicine Department, Medical University, Ul. Mikulicza-Radeckiego 4, 50-368 Wroclaw, Poland
5Pharmaceutical Biochemistry Department, Medical University, ul. Szewska 38/39, 50-139 Wroclaw, Poland

Received 25 October 2011; Accepted 12 December 2011

Academic Editor: Ada Youk

Copyright © 2012 Eugenia Murawska-Ciałowicz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The genotioxic and carcinogenic effect of nickel probably results from its capacity to produce reactive oxygen species (ROS) and disturb the redox balance. The aim of the study was to find out if rats lacking spermatic protamine 2 are less susceptible to Ni(II) than mice. Consequently, the levels of malondialdehyde + 4 hydroxynonenal (MDA+4HDA) − markers of lipid peroxidation, as well as the level of reduced glutathione (GSH) were measured within the rat and mouse testes. Our results showed that the levels of lipid peroxidation markers were elevated in testicular homogenates of intoxicated mice without any changes in rats. GSH level was lower in the group of intoxicated mice comparing to the control without statistically significant changes in rats’ homogenates. Moreover, the level of GSH in the testes of intoxicated mice was lower than in rats. On the basis of our results, it appears that Ni(II) can initiate oxidative stress in the testes of mice but not of rats and can reduce GSH level. Consequently, the antioxidative defense of the testes is reduced. Ni(II) that causes oxidative stress in the testes may also contribute to infertility.