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The Scientific World Journal
Volume 2012, Article ID 398725, 9 pages
http://dx.doi.org/10.1100/2012/398725
Research Article

Role of Laccase and Low Molecular Weight Metabolites from Trametes versicolor in Dye Decolorization

Department of Chemical Engineering, University of Vigo, Isaac Newton Building, Lagoas-Marcosende s/n, 36310 Vigo, Spain

Received 28 October 2011; Accepted 4 January 2012

Academic Editors: G. Galiba and J. Peng

Copyright © 2012 Diego Moldes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The studies regarding decolorization of dyes by laccase may not only inform about the possible application of this enzyme for environmental purposes, but also may provide important information about its reaction mechanism and the influence of several factors that could be involved. In this paper, decolorization of crystal violet and phenol red was carried out with different fractions of extracellular liquids from Trametes versicolor cultures, in order to describe the role of laccase in this reaction. Moreover, the possible role of the low molecular weight metabolites (LMWMs) also produced by the fungus was evaluated. The results confirm the existence of a nonenzymatic decolorization factor, since the nonprotein fraction of the extracellular liquids from cultures of T. versicolor has shown decolorization capability. Several experiments were performed in order to identify the main compounds related to this ability, which are probably low molecular weight peroxide compounds.