Research Article

Modelling the Effects of Ageing Time of Starch on the Enzymatic Activity of Three Amylolytic Enzymes

Figure 1

(a) Changes in the experimental amylolytic activity ( 𝑣 ) data (means ± standard deviations) with the concentration of unaged starch (O) and starches [ 𝑆 ] aged for 24 h (□), 48 h ( ), and 100 h ( ). The curves drawn through the experimental velocity data in (a) were obtained according to the Michaelis-Menten model (1). The curves drawn through the 𝑣 m a x and 𝐾 𝑀 data (as means ± standard errors) for the enzymes BAN (O), San Super (□), and Termamyl ( ) were obtained according to a linear (b), exponential (c), and hyperbolic (d) equation. 𝑌 represents the predicted values for 𝑣 m a x and 𝐾 𝑀 , 𝑎 and 𝑏 are constants, and 𝑡 is the ageing time.
402439.fig.001a
(a)
402439.fig.001b
(b)
402439.fig.001c
(c)
402439.fig.001d
(d)