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The Scientific World Journal
Volume 2012, Article ID 402919, 18 pages
http://dx.doi.org/10.1100/2012/402919
Research Article

Vesiculation from Pseudomonas aeruginosa under SOS

1Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
2Pediatric Biochemistry Laboratory, The University of Texas at San Antonio, San Antonio, TX 78249, USA
3Department of Biology, The University of Texas at San Antonio, San Antonio, TX 78249, USA
4Department of Chemistry, The University of Texas at San Antonio, San Antonio, TX 78249, USA
5RCMI Proteomics, The University of Texas at San Antonio, San Antonio, TX 78249, USA
6Protein Biomarkers Cores, The University of Texas at San Antonio, San Antonio, TX 78249, USA
7Center for Research and Training in the Sciences, The University of Texas at San Antonio, San Antonio, TX 78249, USA
8Division of Hematology and Medical Oncology, Department of Medicine, Cancer Therapy and Research Center, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA

Received 3 October 2011; Accepted 23 October 2011

Academic Editor: Paul Cos

Copyright © 2012 Reshma Maredia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Bacterial infections can be aggravated by antibiotic treatment that induces SOS response and vesiculation. This leads to a hypothesis concerning association of SOS with vesiculation. To test it, we conducted multiple analyses of outer membrane vesicles (OMVs) produced from the Pseudomonas aeruginosa wild type in which SOS is induced by ciprofloxacin and from the LexA noncleavable (lexAN) strain in which SOS is repressed. The levels of OMV proteins, lipids, and cytotoxicity increased for both the treated strains, demonstrating vesiculation stimulation by the antibiotic treatment. However, the further increase was suppressed in the lexAN strains, suggesting the SOS involvement. Obviously, the stimulated vesiculation is attributed by both SOS-related and unrelated factors. OMV subproteomic analysis was performed to examine these factors, which reflected the OMV-mediated cytotoxicity and the physiology of the vesiculating cells under treatment and SOS. Thus, SOS plays a role in the vesiculation stimulation that contributes to cytotoxicity.