Local Treatment of Hand-Foot Syndrome with Uridine/Thymidine: In Vitro Appraisal on a Human Keratinocyte Cell Line HaCaT

Figure 4

MTT test. Comparison of the protective effect of uridine only and uridine together with thymidine in the presence of 7.5 μg 5-FU/mL. The curves represent the course of HaCaT cell viability after 6 days of exposure to 5-FU (7.5 μg/mL) only (red curve) or with protective agents: green (5-FU + uridine 50 μg/mL), blue (5-FU + uridine 50 μg/mL and thymidine 25 μg/mL). The black curve represents control cells in the culture medium. Microphotographs of HaCaT cells stained with May-Grünwald/Giemsa-Romanowski (day 3, objective 10x) demonstrate the resultant state of the outgrowth of the cell population.