The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate

Table 2

Average Olive moment mean values ± (SD) of the amount of DNA damage of Me45 line before and after leachate treatment. Shapiro-Wilk statistic and its probability for testing distributions normality. Normal distribution before transformation (*). Normal distribution after log₁₀ transformation (**).

Time (min)	Before treatment			After treatment
	Olive moment			Olive moment
	Me45_0.1%	Me45_1%	Me45_0.1%	Me45_0.1%	Me45_1%	Me45_10%
control_0		2.50±2.7			1.3±2.15
		𝑃<0.0001			𝑃<0.0001
control_180		3.19±3.12			2.18±4.9
		𝑃=0.0239			𝑃<0.0001
0	55.91±28.07	65.35±19.79	62.11±33.82	9.17±25.35	8.71±17.95	12.31±24.4
		(∗)
	𝑃=0.0004	𝑃=0.2683	𝑃=0.0463	𝑃<0.0001	𝑃<0.0001	𝑃<0.0001
15	54.59±33.05	51.24±34.11	65.57±25.11	9.91±23.61	14.56±33.70	10.03±27.52
			(∗)
	𝑃=0.0016	𝑃=0.0075	𝑃=0.7550	𝑃<0.0001	𝑃<0.0001	𝑃<0.0001
30	48.66±20.37	53.77±16.37	57.92±18.24	8.47±22.43	7.72±24.67	7.82±25.82
	(∗)	(∗)(∗∗)	(∗)(∗∗)
	𝑃=0.4416	𝑃=0.3155	𝑃=0.6724	𝑃<0.0001	𝑃<0.0001	𝑃<0.0001
60	52.26±20.21	30.01±2.00	57.06±17.07	4.44±15.73	3.52±10.03	3.83±9.23
	(∗)		(∗)
	𝑃=0.7725	𝑃<0.0001	𝑃=0.8427	𝑃<0.0001	𝑃<0.0001	𝑃<0.0001
120	32.44±26.46	30.76±19.34	35.23±27.32	3.37±10.37	2.81±4.58	3.06±13.34
	𝑃=0.0009	𝑃<0.0001	𝑃=0.0037	𝑃<0.0001	𝑃<0.0001	𝑃<0.0001
180	28.00±19.93	23.85±11.77	31.50±21.51	4.27±5.78	3.77±13.31	3.89±12.2
	(∗)		(∗)
	𝑃=0.3317	𝑃<0.0001	𝑃=0.5931	𝑃<0.0001	𝑃<0.0001	𝑃<0.0001