Prevalence of Avian Pathogenic Escherichia coli (APEC) Clone Harboring sfa Gene in Brazil

Table 1

The primers used for detection of the various genes by PCR, amplicon size, and references.

Gene	Oligonucleotide primer pairs (5′—3′)	Amplicon (bp)	Reference
papEF	GCAACAGCAACGCTGGTTGCATCAT; AGAGAGAGCCACTCTTATACGGACA	336	[20]
sfa	CTCCGGAGAACTGGGTGCATCTTAC; CGGAGGAGTAATTACAAACCTGGCA	410	[20]
hlyA	AACAAGGATAAGCACTGTTCTGGCT; ACCATATAAGCGGTCATTCCCGTCA	1177	[20]
iucD	TACCGGATTGTCATATGCAGACCGT; AATATCTTCCTCCAGTCCGGAGAAG	602	[20]
cnf1	AAGATGGAGTTTCCTATGCAGGAG; CATTCAGAGTCCTGCCCTCATTATT	498	[20]
crl	TTTCGATTGTCTGGCTGTATG; CTTCAGATTCAGCGTCGTC	250	[21]
csgA	ACTCTGACTTGACTATTACC; AGATGCAGTCTGGTCAAC	200	[21]
tsh	GGGAAATGACCTGAATGCTGG; CCGCTCATCAGTCAGTACCAC	420	[21]
iss	GTGGCGAAAACTAGTAAAACAGC; CGCCTCGGGGTGGATAA	760	[22]
neuS; (kps)	TATAATTAGTAACCTGGGGC; GGCGCTATTGAATAAGACTG	927	[23]