Table of Contents Author Guidelines Submit a Manuscript
The Scientific World Journal
Volume 2012, Article ID 450920, 5 pages
http://dx.doi.org/10.1100/2012/450920
Research Article

Evaluation of the Utility of the Random Amplified Polymorphic DNA Method and of the Semi-Specific PCR to Assess the Genetic Diversification of the Gerbera jamesonii Bolus Line

Department of Ornamental Plants, Warsaw School of Life Sciences, Faculty of Horticulture and Landscape Architecture, Warsaw University of Life Sciences, Nowoursynowska 159, 02776 Warsaw, Poland

Received 13 December 2011; Accepted 3 January 2012

Academic Editor: Kaye Spratt

Copyright © 2012 Zbigniew Rusinowski and Olga Domeradzka. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

An attempt was made to evaluate the utility of a method which employs semi-specific PCR using partially specific primers for the coding sequence (ET) at the exon-intron contact and of the RAPD method to identify eight Polish cultivars of gerbera. It was demonstrated that the PCR method which employs semi-specific primers is as simple and economical as the RAPD method, simultaneously the images of the multiplied by means of the semi-specific PCR method DNA fragments are more complex and polymorphic than those obtained through the RAPD method. The studies of the genetic diversification of Gerbera cultivars employing the aforementioned methods made it possible to conduct a concentration analysis and evaluation of the genetic distance between the lines, manifesting at the same time the superiority of the semi-random PCR method. Moreover, it transpired that the use of mixtures of RAPD primers not always leads to an increase of the number of generated polymorphic bands.