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The Scientific World Journal
Volume 2012, Article ID 454176, 5 pages
Research Article

The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity

Department of Microbiology, Kazan (Volga Region) Federal University, Kremliovskaya Street 18, Kazan 420008, Tatarstan, Russia

Received 31 October 2011; Accepted 2 January 2012

Academic Editor: Marc Graille

Copyright © 2012 Julia Romanova and Maria Filimonova. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Examination of the effects of mononucleotides on Sma nuc endonuclease originated from Gram negative bacterium Serratia marcescens displayed that any mononucleotide produced by Sma nuc during hydrolysis of DNA or RNA may regulate the enzyme activity affecting the RNase activity without pronounced influence on the activity towards DNA. The type of carbohydrate residue in mononucleotides does not affect the regulation. In contrast, the effects depend on the type of bases in nucleotides. AMP or dAMP was classified as a competitive inhibitor of partial type. GMP, UMP, and CMP were found to be uncompetitive inhibitors that suggest a specific site(s) for the nucleotide(s) binding in Sma nuc endonuclease.