Selection of the In Vitro Culture Media Influences mRNA Expression of Hedgehog Genes, Il-6, and Important Genes regarding Reactive Oxygen Species in Single Murine Preimplantation Embryos

Figure 2

Quantitative analysis of mRNA expression of shh, and ihh of single murine blastocyst either cultured in vivo (white) or in vitro in COOK’s Cleavage medium (grey) or in Vitrolife’s G-1 PLUS medium (black) compared to β-actin as housekeeping gene with *𝑃<0.05; **𝑃<0.02; ***𝑃<0.01.