Research Article

Selection of the In Vitro Culture Media Influences mRNA Expression of Hedgehog Genes, Il-6, and Important Genes regarding Reactive Oxygen Species in Single Murine Preimplantation Embryos

Figure 4

Quantitative analysis of mRNA expression of nox, gpx4, gpx1 and prdx2 of single murine blastocyst either cultured in vivo (white) or in vitro in COOK’s Cleavage medium (grey) or in Vitrolife’s G-1 PLUS medium (black) compared to β-actin as housekeeping gene with * 𝑃 < 0 . 0 5 ; ** 𝑃 < 0 . 0 2 ; *** 𝑃 < 0 . 0 1 .
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