Fluorescence Correlation Spectroscopy in Drug Discovery: Study of Alexa532-Endothelin 1 Binding to the Endothelin ETA Receptor to Describe the Pharmacological Profile of Natural Products
Figure 2
Comparison of autocorrelation curves of the Alexa532-ET1 (14.3 nM) binding to the ETA receptor on living A10 cells. Control experiments (blue), coincubation with 1 μM BQ-123 (green) leads to an autocorrelation curve similar to that of the free ligand (red) indicating the displacement of the bound Alexa532-ET1.
