Research Article

Induction of MAP Kinase Homologues during Growth and Morphogenetic Development of Karnal Bunt (Tilletia indica) under the Influence of Host Factor(s) from Wheat Spikes

Table 2

Determination of morphological variation and sporidial count of T. indica isolate grown in presence and absence of host factor(s).

Days of cultureIn absence of host factor(s)In presence of host factor(s)
Morphological variationSporidial count (Number of sporidia/mL)Morphological variationSporidial count (number of sporidia/mL)

7thThin long, interwoven vegetative mycelia with less septation and nucleiNilThick, comparatively small vegetative mycelia with extensive septation and multiple nucleiNil

14thThickened, branched mycelia which produces secondary sporidia at the tip of lateral small branches 1 . 8 × 1 0 3 Thickened, small vegetative myceliaNil

21stAcicular, tapered, banana-shaped high number sporidia and sporogenous mycelia seen occasionally 3 . 9 × 1 0 9 Thickened mycelia which produce few banana-shaped allantoid sporidia 4 . 1 × 1 0 7

30thCrumpled sporogenous mycelia with short sporogenous hypal branches, terminal/intercalary rounding up of hypal cells, sometimes in chain. Few small chlamydospore like structures 8 . 2 × 1 0 9 Enlarged and many sporogenous mycelia and less hyphal branches and not much chlamydospore formation 1 . 3 × 1 0 8