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The Scientific World Journal
Volume 2012, Article ID 543536, 9 pages
http://dx.doi.org/10.1100/2012/543536
Research Article

A Novel Murine Model for the In Vivo Study of Transdermal Drug Penetration

1Department of Dermatology and Allergology, University of Szeged, 6720 Szeged, Hungary
2Institute of Surgical Research, University of Szeged, 6720 Szeged, Hungary
3Department of Pharmaceutical Technology, University of Szeged, 6720 Szeged, Hungary
4Department of Pharmacodynamics and Biopharmacy, University of Szeged, 6720 Szeged, Hungary
5Dermatological Research Group of the Hungarian Academy of Sciences and the University of Szeged, 6720 Szeged, Hungary

Received 10 October 2011; Accepted 2 November 2011

Academic Editors: P. Danckwerts and D. Quintanar-Guerrero

Copyright © 2012 Gábor Erős et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Enhancement of the transdermal penetration of different active agents is an important research goal. Our aim was to establish a novel in vivo experimental model which provides a possibility for exact measurement of the quantity of penetrated drug. The experiments were performed on SKH-1 hairless mice. A skin fold in the dorsal region was fixed with two fenestrated titanium plates. A circular wound was made on one side of the skin fold. A metal cylinder with phosphate buffer was fixed into the window of the titanium plate. The concentration of penetrated drug was measured in the buffer. The skin fold was morphologically intact and had a healthy microcirculation. The drug appeared in the acceptor buffer after 30 min, and its concentration exhibited a continuous increase. The presence of ibuprofen was also detected in the plasma. In conclusion, this model allows an exact in vivo study of drug penetration and absorption.