Research Article

Inactivation of the Catalytic Subunit of cAMP-Dependent Protein Kinase A Causes Delayed Appressorium Formation and Reduced Pathogenicity of Colletotrichum gloeosporioides

Table 1

Oligonucleotide primers used in this study.

NameDescriptionSequence

c-75FForward primer to amplify 75 bp CgPKAC partial gene during real-time RT-PCR5′-GGTCTCATAAATCATGTTTGCACTG-3′
c-75RReverse primer to amplify 75 bp CgPKAC partial gene during real-time RT-PCR5′-CGTCATTGCTTTCCTATCCAT-3′
18SF2Forward primer to amplify 101 bp 18S rDNA partial gene during real-time RT-PCR5′-CAGCGAAATGCGATAAGTAATG-3′
18SR2Reverse primer to amplify 101 bp 18S rDNA partial gene during real-time RT-PCR5′-GCAGAGCTTGAGGGTTGAAAT-3′
CGFPrimer used for 5′-RACE PCR amplification5′-GTCCGACAGACGAAGGGGAAATAC-3′
CGRPrimer used for 3′-RACE PCR amplification5′-CCACGGATTTGTTGTAGCCCTTGT-3′
TSP1Template-specific primer used in DNA walking for CgPKAC regulatory region amplification5′-GCAGCGAGAAGAGTTTCACCAC-3′
TSP2Template-specific primer used in DNA walking for CgPKAC regulatory region amplification5′-GTATTTCCCCTTCGTCTGTCGG-3′
TSP3Template-specific primer used in DNA walking for CgPKAC regulatory region amplification5′-GATTAGGAGGATGGATGGTGAC-3′
PKACpN5-FForward primer to amplify 695 bp 5′ region of CgPKAC for construction into hygromycin cassette5′-CGCTCACATTGGTACCGGTTCC-3′
PKACpN5-RReverse primer to amplify 695 bp 5′ region of CgPKAC for construction into hygromycin cassette5′-ACGGAAGGATCCAGGGCAATCA-3′
PKACpN3-FForward primer to amplify 376 bp 5′ region of CgPKAC for construction into hygromycin cassette5′-ACGGGCATGCGGCCGCGGTTTCT-3′
PKACpN3-RReverse primer to amplify 376 bp 5′ region of CgPKAC for construction into hygromycin cassette5′-CCTTTGAAGCGCATGCCCGACCC-3′