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Name | Description | Sequence |
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c-75F | Forward primer to amplify 75 bp CgPKAC partial gene during real-time RT-PCR | 5′-GGTCTCATAAATCATGTTTGCACTG-3′ |
c-75R | Reverse primer to amplify 75 bp CgPKAC partial gene during real-time RT-PCR | 5′-CGTCATTGCTTTCCTATCCAT-3′ |
18SF2 | Forward primer to amplify 101 bp 18S rDNA partial gene during real-time RT-PCR | 5′-CAGCGAAATGCGATAAGTAATG-3′ |
18SR2 | Reverse primer to amplify 101 bp 18S rDNA partial gene during real-time RT-PCR | 5′-GCAGAGCTTGAGGGTTGAAAT-3′ |
CGF | Primer used for 5′-RACE PCR amplification | 5′-GTCCGACAGACGAAGGGGAAATAC-3′ |
CGR | Primer used for 3′-RACE PCR amplification | 5′-CCACGGATTTGTTGTAGCCCTTGT-3′ |
TSP1 | Template-specific primer used in DNA walking for CgPKAC regulatory region amplification | 5′-GCAGCGAGAAGAGTTTCACCAC-3′ |
TSP2 | Template-specific primer used in DNA walking for CgPKAC regulatory region amplification | 5′-GTATTTCCCCTTCGTCTGTCGG-3′ |
TSP3 | Template-specific primer used in DNA walking for CgPKAC regulatory region amplification | 5′-GATTAGGAGGATGGATGGTGAC-3′ |
PKACpN5-F | Forward primer to amplify 695 bp 5′ region of CgPKAC for construction into hygromycin cassette | 5′-CGCTCACATTGGTACCGGTTCC-3′ |
PKACpN5-R | Reverse primer to amplify 695 bp 5′ region of CgPKAC for construction into hygromycin cassette | 5′-ACGGAAGGATCCAGGGCAATCA-3′ |
PKACpN3-F | Forward primer to amplify 376 bp 5′ region of CgPKAC for construction into hygromycin cassette | 5′-ACGGGCATGCGGCCGCGGTTTCT-3′ |
PKACpN3-R | Reverse primer to amplify 376 bp 5′ region of CgPKAC for construction into hygromycin cassette | 5′-CCTTTGAAGCGCATGCCCGACCC-3′ |
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