Genetic Dissection of Sympatric Populations of Brown Planthopper, Nilaparvata lugens (Stål), Using DALP-PCR Molecular Markers

Table 3

Frequency of presence of bands (%) markers in Nilaparvata spp. obtained from three DALP primers.

Primer	Marker (M. Wt.)	UPM		Tanjung Karang		Melaka		Perak		Sabah		Cameron High lands
		N. lugens (Rice)	N. lugens (Leersia)	N. lugens (Rice)	N. lugens (Leersia)	N. lugens (Rice)	N.lugens (Leersia)	N. lugens (Rice)	N.lugens (Leersia)	N. lugens (Rice)	N.lugens (Leersia)	N.bakeri (Leersia)
	DALP231.1 (2.15 kb)	10	10	0	0	10	20	10	0	45	10	0
	DALP231.2 (2.0 kb)	0	25	0	0	40	30	15	0	50	10	30
	DALP231.3 (1.9 kb)	25	0	0	0	40	35	15	10	25	10	0
	DALP231.4 (1.8 kb)	100	65	100	90	100	90	100	80	70	100	80
	DALP231.5 (1.7 kb)	100	100	100	100	100	100	100	100	100	100	90
	DALP231.6 (1.6 kb)	100	100	100	100	100	100	100	100	100	100	0
	DALP231.7 (1.4 kb)	60	100	100	80	50	100	100	60	70	100	90
	DALP231.8 (1.3 kb)	100	100	100	100	100	100	100	100	100	100	100
	DALP231.9 (1.2 kb)	100	100	100	100	100	100	100	100	100	100	0
	DALP231.10 (1.0 kb)	0	60	0	50	0	40	0	60	0	40	100
DALP231	DALP231.11 (0.9 kb)	0	10	10	0	0	20	0	0	0	30	0
	DALP231.12 (0.85 kb)	30	40	25	50	30	30	20	30	40	35	80
	DALP231.13 (0.75 kb)	0	0	0	0	0	0	0	0	0	0	100
	DALP231.14 (0.70 kb)	0	0	0	0	0	0	0	0	0	0	100
	DALP231.15 (0.60 kb)	0	0	0	0	0	0	0	0	30	30	0
	DALP231.16 (0.50 kb)	0	0	0	0	0	0	0	0	0	0	100
	DALP231.17 (0.40 kb)	100	100	100	100	100	100	100	100	100	100	100
	DALP231.18 (0.30 kb)	100	100	100	100	100	100	100	100	100	100	0
	DALP231.19 (0.25 kb)	100	100	100	100	100	100	100	100	100	100	100
	DALP231.20 (0.20 kb)	80	60	100	100	100	100	100	100	100	100	0
	DALP231.21 (0.10 kb)	100	100	100	100	100	100	100	100	100	100	100
	DALP231.22 (0.09 kb)	0	0	0	0	0	0	0	0	0	0	100
	DALP234.1 (2.0 kb)	100	100	100	100	100	100	100	100	100	100	0
	DALP234.2 (1.9 kb)	100	100	100	100	100	100	100	100	100	100	0
	DALP234.3 (1.8 kb)	20	50	25	50	20	50	20	30	30	50	0
	DALP234.4 (1.75 kb)	0	0	0	0	0	0	0	0	0	0	100
	DALP234.5 (1.70 kb)	100	100	100	100	100	100	100	100	100	100	0
	DALP234.6 (1.65 kb)	0	70	0	40	0	40	0	60	0	40	100
DALP234	DALP234.7 (1.6 kb)	0	10	30	0	0	20	0	0	0	30	0
	DALP234.8 (1.5 kb)	40	40	60	50	30	30	20	30	40	35	100
	DALP234.9 (1.45 kb)	0	0	0	0	0	0	0	0	0	0	100
	DALP234.10 (1.3 kb)	100	100	100	100	100	100	100	100	100	100	100
	DALP234.11 (1.25 kb)	0	0	0	0	0	0	0	0	30	30	0
	DALP234.12 (1.10 kb)	0	0	0	0	0	0	0	0	0	0	100
	DALP234.13 (1.0 kb)	100	100	100	100	100	100	100	100	100	100	100
	DALP234.14 (0.9 kb)	100	100	100	100	100	100	100	100	100	100	0
	DALP234.15 (0.85 kb)	100	100	100	100	100	100	100	100	100	100	100
	DALP234.16 (0.7 kb)	100	100	100	100	100	100	100	100	100	100	0
	DALP234.17 (0.65 kb)	90	90	100	100	100	100	90	100	100	90	100
	DALP234.18 (0.5 kb)	0	0	0	0	0	0	0	0	0	0	100
	DALP234.19 (0.35 kb)	40	50	100	100	100	100	100	100	80	90	100
	DALP234.20 (0.30 kb)	80	20	80	70	60	40	50	90	70	80	100
	DALP234.21 (0.15 kb)	40	50	90	100	80	100	100	100	80	90	100
	DALP235.1 (2.2 kb)	5	10	10	8	0	10	9	20	40	35	0
	DALP235.2 (2.1 kb)	100	100	100	100	100	100	100	100	100	100	100
	DALP235.3 (2.0 kb)	100	100	100	100	100	100	100	100	100	100	0
	DALP235.4 (1.9 kb)	100	100	100	100	100	100	100	100	100	100	100
	DALP235.5 (1.8 kb)	10	40	15	50	20	35	16	30	10	50	0
	DALP235.6 (1.75 kb)	0	0	0	0	0	0	0	0	0	0	100
	DALP235.7 (1.70 kb)	100	100	100	100	100	100	100	100	100	100	0
	DALP235.8 (1.65 kb)	0	60	0	50	0	40	0	60	0	40	100
	DALP235.9 (1.6 kb)	0	10	10	0	0	20	0	0	0	30	0
	DALP235.10 (1.5 kb)	30	40	25	50	30	30	20	30	40	35	100
	DALP235.11 (1.4 kb)	0	0	0	0	0	0	0	0	0	0	100
	DALP235.12 (1.3 kb)	0	0	0	0	0	0	0	0	0	0	100
	DALP235.13 (1.2 kb)	0	0	0	0	0	0	0	0	30	30	0
	DALP235.14 (1.15 kb)	0	0	0	0	0	0	0	0	0	0	100
	DALP235.15 (1.0 kb)	100	100	100	100	100	100	100	100	100	100	100
DALP235	DALP235.16 (0.9 kb)	100	100	100	100	100	100	100	100	100	100	100
	DALP235.17 (0.8 kb)	100	100	100	100	100	100	100	100	100	100	100
	DALP235.18 (0.7 kb)	100	100	100	100	100	100	100	100	100	100	0
	DALP235.19 (0.6 kb)	100	100	100	100	100	100	100	100	100	100	100
	DALP235.20 (0.5 kb)	0	0	0	0	0	0	0	0	0	0	100
	DALP235.21 (0.35 kb)	30	50	100	100	100	100	100	100	80	90	70
	DALP235.22 (0.30 kb)	90	20	80	70	60	40	50	90	70	80	100
	DALP235.23 (0.20 kb)	60	90	50	30	80	100	90	60	40	75	100
	DALP235.24 (0.15 kb)	100	100	80	100	100	100	100	100	90	100	90
	DALP235.25 (0.10 kb)	100	100	100	100	100	100	100	100	100	100	100
Total Number of markers		45	51	46	46	45	52	46	47	49	55	41
Number of polymorphic markers		17	23	14	14	13	18	13	15	21	25	7
% Polymorphic markers		37.77	45.09	30.43	30.43	28.88	34.61	28.26	31.91	42.85	45.45	17.08
Overall number of markers based on the populations of N. lugens												68
Overall number of polymorphic markers based on the populations of N. lugens												29
Overall % poymorphism based on the populations of N. lugens												42.64

Frequency based upon 20 individuals per population.