NOD2 Agonist Promotes the Production of Inflammatory Cytokines in VSMC in Synergy with TLR2 and TLR4 Agonists

Figure 3

Proliferation ability of VSMC in response to MDP alone or in synergy with LPS and PAM3. MTT assay was done to test the proliferative activity of VSMC. MDP, LPS, or PAM3 alone can stimulate VSMC proliferation ability (MDP: 0.87 ± 0.05; LPS: 0.94 ± 0.06; PAM3: 0.84 ± 0.06; control: 0.72 ± 0.02; ^a𝑃<0.05 versus control, 𝑛=6). The combination of MDP with LPS or PAM3 got more activated proliferation ability (MDP + LPS: 1.25 ± 0.04; MDP + PAM3: 1.19 ± 0.05; ^b𝑃<0.05 versus MDP; ^c𝑃<0.05 versus LPS; ^d𝑃<0.05 versus PAM3; 𝑛=6).