Research Article
Shiga Toxin-Producing Escherichia coli Isolated from Bovine Mastitic Milk: Serogroups, Virulence Factors, and Antibiotic Resistance Properties
Table 1
Primers used for detection of virulence genes in Shiga toxin-producing Escherichia coli isolated from bovine mastitis.
| Virulence factor | Primers name | Primer sequences (5′-3′) | Product size (bp) | Reference |
| Shiga toxin 1 (stx1)
| Stx1f | AAATCGCCATTCGTTGACTACTTCT | 366
|
[20] | Stx1r | TGCCATTCTGGCAACTCGCGATGCA | Shiga toxin 2 (stx2)
| Stx2f | CGATCGTCACTCACTGGTTTCATCA | 282
|
[20] | Stx2r | GGATATTCTCCCCACTCTGACACC | Enteropathogenic attachment and effacement (eaeA) | EAE1 | TGCGGCACAACAGGCGGCGA | 629
|
[21] | EAE2 | CGGTCGCCGCACCAGGATTC | Haemolysin (ehly) | Hly F | CAATGCAGATGCAGATACCG | 432 |
[22] | Hly R | CAGAGATGTCGTTGCAGCAG |
|
|