Evolutionary Relationship between Two Firefly Species, Curtos costipennis and C. okinawanus (Coleoptera, Lampyridae), in the Ryukyu Islands of Japan Revealed by the Mitochondrial and Nuclear DNA Sequences

Table 2

PCR primers used in this study shown in 5′–3′ direction.

Gene	Name^(1)	Sequence
COI-COII	FFMT2210F	TAC CAG GAT TTG GTA TAA TTT CTC AT
	FFMT3578R	GGA TAG TTC ATG AGT GGA TTA CAT C
	FFMT3140R	ATT GTT CTA TTA AAG GTG AAA TTC T
16S rDNA	AAMT12948F	ATC CAA CAT CGA GGT CGC AAA CT^(2)
	FFMT13911R	GTA GTT TTG TAC CTT GTG TAT CAG GGT
ITS2	ITS2F	TGT GAA CTG CAG GAC ACA TG
	ITS2R	CCT GTT CGC TCG CAG CTA CT
	FFITS2F	GGT GAG CTC GTC CCC GCA TCG
	FFITS2R	GTG TAA TAT CAT TTG ATA TCG

⁽¹⁾Numerals in the name of primers for COI-COII and 16S rDNA indicate nucleotide position in the total mtDNA sequence of Drosophila yakuba [8] corresponding to the 5′ end of the primer.
⁽²⁾AAMT12948F was designed in our previous study [9].