Apoptosis of Epithelial Cells and Macrophages due to Nonpigmented Serratia marcescens Strains
Table 1
Apoptotic index of HEp-2 and J774 cells infected with S. marcescens strains at 24 h and 48 h, cell-contact and extracellular hemolytic activity, cell-contact cytotoxic activity, and invasion ability.
Strain no.
Apoptotic index1 (HEp-2)
Apoptotic index (J774)
Hemolytic activity2
Cell-contact cytotoxicity3 [%]
Invasion index4 [%]
Cell contact
Extracellular
MPU S42
49.1/71.7
57.6/75.3
74.3
0.95
79.1
57.3
MPU S29
48.1/70.6
53.7/74.3
71.3
7.4
76.8
59.8
MPU S6
47.9/69.1
39.7/55.1
61.2
10.7
69.1
53.2
MPU S1
54.3/68.4
6.8/25.7
0.98
16.8
14.8
13.4
MPU S3
44.7/64.1
5.6/33.3
0.81
8.3
25.4
11.2
MPU S12
43.1/66.9
34.9/41.6
65.9
14.5
60.9
43.2
MPU S34
42.1/59.6
52.1/73.1
59.7
2.9
65.7
49.4
MPU S30
41.7/63.4
51.6/59.1
58.3
5.1
60.2
35.7
MPU S31
38.7/68.1
18.3/54.6
64.8
6.4
81.3
54.4
MPU S23
37.1/71.7
34.8/41.9
49.6
0.75
52.9
39.1
MPU S11
36.7/67.2
42.6/48.9
0.70
17.2
12.6
12.7
MPU S33
35.1/48.1
55.8/75.3
68.9
1.5
72.6
48.2
MPU S21
34.1/61.2
31.6/36.8
8.0
0.70
34.8
11.7
MPU S7
34.1/65.4
41.8/45.7
38.7
12.9
61.2
31.2
MPU S5
33.4/53.8
39.9/42.1
4.1
11.6
4.8
6.3
MPU S22
31.3/64.2
46.1/52.8
15.6
15.7
28.4
12.4
MPU S28
29.6/43.7
27.6/60.4
17.9
4.1
41.8
11.7
MPU S27
28.4/37.9
14.3/48.3
5.1
2.4
6.1
6.3
MPU S14
27.1/59.6
20.1/29.1
4.4
6.9
9.1
6.1
MPU S4
26.8/38.1
22.7/30.6
3.1
9.1
4.1
5.8
MPU S26
24.4/54.1
47.5/57.3
45.3
3.4
59.1
41.2
MPU S36
24.1/49.6
45.8/57.1
21.7
0.81
45.1
35.7
MPU S13
23.9/58.1
43.9/54.6
12.8
2.1
49.6
28.4
MPU S41
23.6/56.1
16.8/28.6
7.6
4.5
3.7
7.4
MPU S37
23.1/51.4
48.6/59.1
41.2
5.7
57.3
28.9
MPU S35
22.8/40.8
9.4/27.3
6.8
4.7
6.4
8.1
MPU S20
22.1/41.4
44.1/48.6
31.7
1.2
37.2
17.4
MPU S18
21.4/41.4
40.4/55.8
34.2
3.1
53.1
8.7
MPU S2
19.3/29.4
11.7/26.1
3.7
13.1
4.6
1.7
MPU S15
14.1/28.7
29.1/31.9
28.2
3.8
52.7
11.3
1The percentage mean of apoptotic cells at 24 and 48 h. 2The percentage of total hemolysis, compared to 100% lysis in distilled water was performed 4 h after infection by using a suspension of 1% human erythrocytes. 3The percentage of cytotoxicity was determined 4 h after infection by MTT assay. 4The percentage of number of internalized bacteria per 100 HEp-2 cells in compare with number of adhering bacteria. Values correspond to the means from two experiments in triplicate.
