The Scientific World Journal / 2012 / Article / Tab 1

Research Article

Apoptosis of Epithelial Cells and Macrophages due to Nonpigmented Serratia marcescens Strains

Table 1

Apoptotic index of HEp-2 and J774 cells infected with S. marcescens strains at 24 h and 48 h, cell-contact and extracellular hemolytic activity, cell-contact cytotoxic activity, and invasion ability.

Strain no. Apoptotic index1 (HEp-2) Apoptotic index (J774) Hemolytic activity2 Cell-contact cytotoxicity3 [%] Invasion index4 [%]
Cell contactExtracellular

MPU S4249.1/71.757.6/75.374.30.9579.157.3
MPU S2948.1/70.653.7/74.371.37.476.859.8
MPU S647.9/69.139.7/55.161.210.769.153.2
MPU S154.3/68.46.8/25.70.9816.814.813.4
MPU S344.7/64.15.6/33.30.818.325.411.2
MPU S1243.1/66.934.9/41.665.914.560.943.2
MPU S3442.1/59.652.1/73.159.72.965.749.4
MPU S3041.7/63.451.6/59.158.35.160.235.7
MPU S3138.7/68.118.3/54.664.86.481.354.4
MPU S2337.1/71.734.8/41.949.60.7552.939.1
MPU S1136.7/67.242.6/48.90.7017.212.612.7
MPU S3335.1/48.155.8/75.368.91.572.648.2
MPU S2134.1/61.231.6/36.88.00.7034.811.7
MPU S734.1/65.441.8/45.738.712.961.231.2
MPU S533.4/53.839.9/42.14.111.64.86.3
MPU S2231.3/64.246.1/52.815.615.728.412.4
MPU S2829.6/43.727.6/60.417.94.141.811.7
MPU S2728.4/37.914.3/48.35.12.46.16.3
MPU S1427.1/59.620.1/29.14.46.99.16.1
MPU S426.8/38.122.7/30.63.19.14.15.8
MPU S2624.4/54.147.5/57.345.33.459.141.2
MPU S3624.1/49.645.8/57.121.70.8145.135.7
MPU S1323.9/58.143.9/54.612.82.149.628.4
MPU S4123.6/56.116.8/28.67.64.53.77.4
MPU S3723.1/51.448.6/59.141.25.757.328.9
MPU S3522.8/40.89.4/27.36.84.76.48.1
MPU S2022.1/41.444.1/48.631.71.237.217.4
MPU S1821.4/41.440.4/55.834.23.153.18.7
MPU S219.3/29.411.7/26.13.713.14.61.7
MPU S1514.1/28.729.1/31.928.23.852.711.3

1The percentage mean of apoptotic cells at 24 and 48 h. 2The percentage of total hemolysis, compared to 100% lysis in distilled water was performed 4 h after infection by using a suspension of 1% human erythrocytes. 3The percentage of cytotoxicity was determined 4 h after infection by MTT assay. 4The percentage of number of internalized bacteria per 100 HEp-2 cells in compare with number of adhering bacteria. Values correspond to the means from two experiments in triplicate.

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